This study is aimed at assessing the patients’ waiting time in the neurology outpatient department of a tertiary health care institution in North East India. This is a prospective observational study where duration of patients’ waiting time, doctor consultation time and total waiting time were noted and recorded for each patient. The participants were enrolled randomly in this study and total number of participants was 80 including men and women from different age ranges. The results included data records of (1) The mean waiting time which was found to be 110.86 minutes (2) Mean consultation time was 5.34 minutes and (3) Mean total waiting time was found to be 116.20 minutes. From the findings of this study, it can be concluded that efforts need to be taken to reduce the long waiting time; which is identified by the WHO as an important index for determining the quality and satisfactory health services; in order to help improve patient’s satisfaction, winning their trust as well as their willingness and promptness to acquire basic healthcare needs and services.
Amatoxins commonly found in Amanita phalloides is the main constituents of toxins present in most toxic mushroom
specimens containing α-amanitin,β-amanitin,γ-amanitin,ε-amanitin,amanin,amaninamide,amanullin,amanullinic acid,
and proamanullin.RP-HPLC analysis of toxins Chromatography:The method of Ismail Yilmaz et.al,is followed using C18
(Agilent Technologies) at UV detection 303nm for amatoxins and 291nm for phallotoxins.The mobile phase in isocratic
pump with a flow rate of 1ml/min consisting of 0.05M ammonium acetate (pH 5.5 with acetic acid) and acetonitrile
(90:10v/v).The geographical variations determine the content of the toxins and it brings a landmark to create awareness
to the community for such mushroom specimens.We hope that analyzing the toxin content in the coming years will be of
great service to the Physician and the community as well.
Flavonoids are secondary plant metabolites normally found as pigmented compounds in plants. Quercetin and rutin are two
important and commonly found flavonoids in nature and exhibit wide pharmacological effects such as antioxidant, anticarcinogenic,
antiviral, anti-inflammatory, antidiabetic, and hepatoprotective activities as well as antimicrobial activity. In this study, quercetin and
rutin content is being quantified in the plant extracts of Centella asiatica and Houttuynia cordata and considerable amounts of these
two flavonoids were depicted. A single beam UV – Spectrophotometer was used to measure the absorbance of the standard as well
as test solutions. Calibration curves were constructed for standard quercetin and rutin in such a way that x-axis denotes concentration
and the y-axis denotes the absorbance. The calibration curves showed linearity at concentrations 5-25 ?g /ml of quercetin and rutin
respectively with a good correlation coefficient (r) of 0.99 for both the curves. The absorbance of the two test extracts was obtained
from the calibration curve and respective concentrations of quercetin and rutin for the two extracts were calculated. The amount of
quercetin and rutin present was expressed as Total Flavonoid Content (TFC) i.e. the amount of the flavonoid in ?g present per mg of
the respective plant extract. The quercetin content in both the plant extracts was found to be more (315.8 in Houttuynia cordata;
487.6 in Centella asiatica) than the content of rutin (152.2 in Houttuynia cordata; 171.0 in Centella asiatica).
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