In this experiment, sub1A quantitative trait loci (sub1A QTL) of rice were evaluated for dehydration responses through different aspects of cellular responses. Through variations of dehydration exposure, rice seedlings recorded a significant increase in superoxide (O2.-) and hydrogen peroxide (H2O2), the former by 1.80 fold and the latter by 2.10 fold. Nicotinamide adenine dinucleotide phosphate oxidase activity fairly correlated with lipid peroxidation (r = 1.96). Both 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) had similar IC50 values over the control at p ≤ 0.05. Reducing power of the extract had 1.31 fold increase. The antioxidant moieties like total phenolics and flavonoids were 1.04 and 1.23 fold upregulated under stress. On the other hand anthocyanin and glutathione (GSH) did not vary much under stress except at maximum duration of stress. Superoxide dismutase (SOD) was initially stable but maximized at 8 days by 1.30 fold increase. On the contrary, guaiacol peroxidase (GPX) was seen to be downregulated by 40.94% all through the days of stress. Catalase (CAT) activity followed a similar trend, but was not significant as compared to control.
The present investigation embodies with some distinct physiological responses of rice seedlings bearing sub1A allele when cultured under soil moisture deficit condition. In order to estimate the extent of variation of sub1A al-How to cite this paper:
Two chili cultivars, i.e., cv. Bullet and cv. Tejaswini, were evaluated on postharvest related ripening characteristics with varying durations under hydrogen peroxide, putrescine and silver treatments. The reducing sugar was inversely related to the maximum values at 7 days of ripening. Silver and putrescine were the most regulatory in terms of changing of the total carbohydrate content as compared to hydrolysis of the total reducing sugar. Regarding pectin methylesterase activity, both chilies were consistent, regardless of the number of days of incubation. Still, putrescine and silver were significant contributors to variations in cv. Bullet and cv. Tejaswani. For the pigment content, lycopene and chlorophyll increased in a linear manner, although these treatments significantly varied over time. Hydrogen peroxide and putrescine were responsible for the maximum accumulation of lycopene for both the cultivars, whereas, only cv. Tejaswani displayed maximum carotenoid for putrescine. Silver for both chili varieties was the most inhibitory for lycopene and carotenoid content. Superoxide had a good impact on the accumulation of lipid peroxides, irrespective of the chili variety. The maximum accumulation of lipid peroxide was recorded at seven days of treatment. Phenolics and flavonoids were in decreasing order for both the chili varieties, progressing through the days of the study period in a similar manner. Silver was the main contributor to variations in the phenolics and flavonoid contents in cv. Tejaswani. The solubilization of total carbohydrate into reducing sugar was in an inverse relationship, with the maximum values being reached at 7 days of ripening.
In the present experiment, Zea mays L. was evaluated for its C4 modality through oxidative stress responses. Moreover, under variable concentrations (0, 50, 100µM) of hydrogen peroxide, the impacts were variable and modulated with titanium dioxide nanoparticle (TiO2-NP). Plants scored with elevated concentrations of H2O2 and superoxide (O2.-) maximum by 1.31 & 1.52-fold respectively over 0µM H2O2. TiO2-NP recovered those maximums with 8.69 & 7.89% as compared to control plants. The effects of H2O2 were moderated with malondialdehyde and carbonyl content by peak values 7.45 & 5.91% under TiO2-NP treatment respectively. A depletion in proline content recorded under H2O2 stress but recovered through highest 1.23-fold with TiO2-NP application. TiO2-NP recorded NADP-ME activities up-regulated in concentration gradient of H2O2. Oxidative exposure was also documented by in-vivo detection of H2O2 and O2.- by 3,3′-diaminobenzidine and nitroblue tetrazolium staining. For the anti-oxidative cascades superoxide dismutase activity had induced by 1.42-fold under H2O2 when pre-treated with TiO2-NP. However, glutathione reductase activity was differentially modulated under H2O2 through interaction with TiO2-NP. Activity of guaiacol peroxidase was significantly up-regulated by 1.07-fold when TiO2-NP applied. The inductions of oxidative stress recorded more evident when a distinct polymorphism for GPX protein resolved variations in band intensities, but not in number. Activity of catalase experience a down-regulated trend all through the H2O2 exposure and had significant sensitivity to TiO2-NP. The results depict the nature and reactivity of C4 modules under oxidative stress that would be the selection pressures and that might be modulated with TiO2-NP application.
Rice genotype (cv. Swarna Sub1) was compared with cv. Swarna with respect to salinity stress. The modality of sub1A traits, which is primarily linked to submergence tolerance, was monitored as to its sensitivity to salinity. Proline and electrolyte leakage were not significantly changed in cv. Swarna Sub1 as compared to cv. Swarna. However, the effects of silver ions through individual, hydroponics and pre-treatment distinctly varied between two cultivars. Silver absorption distinctly varied with 45.01-fold rises in cv. Swarna against control, whereas for sodium cv. Swarna Sub1 scored the highest accumulation by 3.03-fold. Potassium depletion in plants recorded a significant down-regulation for both the genotypes, maximally under salinity by 43.78 and 22.33%, respectively. Plants exhibited a significant suppression of shoot and root length which finally manifested in dry matter accumulation by 93.95 and 89.24%, respectively, for cv. Swarna and Swarna Sub1 under pre-treatment. Chlorophyll content significantly varied among the treatments as well as between the cultivars. Carotenoids and lycopene though depleted but had not any significant variation between the cultivars. NADP(H)-oxidase was distinctly overexpressed for cv. Swarna Sub1 by 1.16-fold under salinity than cv. Swarna (1.22-fold). Still, pre-treatment with silver has maximally induced superoxide content establishing oxidative sensitivities with a peak rise of 3.07 and 2.44-fold for two genotypes. Therefore, the significant traits linked to sub1A QTL would be the selection markers or to overexpress in susceptible genotypes under the condition of salinity for better sustenance.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.