Background: Most of the medicinal plants are available as wild or weeds in the world including Pakistan. It was reported by many researchers that Pakistan is rich with medicinal flora based upon the surveys conducted in the Northern areas but still there are some areas other than Northern parts in Pakistan that has not been studied so far. There is a need to explore the medicinal flora from neglected area where most of the people still rely on local medicinal plants to cure their diseases by means of indigenous knowledge. District Gujrat is among those area that has not been explored to study the medicinal flora.Methods: Surveys were conducted to collect the indigenous knowledge and medicinal significance of seasonal weeds in Gujrat, Punjab, Pakistan in winter 2018 and summer 2019. Information collected through questionnaire and interviews.Results: Weeds have much importance among local inhabitants to cure many diseases as respiratory disorders, kidney and liver disorders, muscle and skeletal disorders, ear, nose and throat problems, dermatological disorders, fever, diabetes, cancer etc. The plant parts and its percentage used by local inhabitants were leaves (58%), fruits (36%), seeds (25%), whole plant (34%), roots (32%), milky latex (1%), flowers (8%) and stem (3%). The most important families were Asteraceae, Poaceae, Fabaceae, Polygonaceae and Solanaceae. Cichorium intybus L. (Asteraceae) had 100% Fidelity Level (FL) value as liver tonic and blood purifier. Highest 0.76 Informant Consensus Factor (ICF) values were noted against stomach, gastric, intestinal and digestive problems. Achyranthes aspera L. (Amaranthaceae) showed the highest Fidelity Level (FL) values that was 93% used gastrointestinal disorders and menstrual pain.Conclusions: It was concluded that the area of Gujrat, Punjab, Pakistan is a rich source of important medicinal weeds that needs to be utilized for useful medicinal purpose. There is need to create awareness among the farmers and local people about the efficacy of weeds.

SummaryIntroduction: Medicinal plants are the natural source of medicines used in treatment of many diseases among local communities of various countries. It is also the raw material for the pharmaceutical industry. Pakistan is a country with a variety of medicinal plants, comprehensively studied in some areas, while others still need more studies of their flora.Objectives: The study was performed to catalogue the flora of district Gujrat, Punjab, Pakistan and to assess its ethnomedicinal importance.Methods: A field survey was conducted in the Gujrat district (including Sarai Alamgir, Gujrat and Kharian) in 2019–2020 to collect the flora. Nearly 200 people of various age groups were interviewed and the questionnaire was filled, the gender and professions of the participants were also noted.Results: The plant species collected belonged to 32 different Angiosperm families i.e. Poaceae, Brassicaceae, Fabaceae, Asteraceae, Moraceae, Amaranthaceae, Apiaceae, Myrtaceae, Rutaceae, Solanaceae, Malvaceae, Euphorbiaceae and Lamiaceae. The obtained material consisted of 30 herbs, 20 shrubs and 22 trees. The floral parts used most often were leaves (63%) followed by: flower (41%), fruit (28%), stem (16%), seed (16%), bark (14%), root (13%), whole plant (12%), underground part (3%) and latex (3%). According to the study, the highest RFC (Relative Frequency of Citation) values were 0.1 in Cassia fistula L. (0.25) and the smallest RFC values were observed in Stellaria media (L.) Vill. – 0.025. The highest UV (Use Values) were noted for Achyranthes aspera L. at 0.9. In disease category, the highest value (0.67) of ICF (Informant Consensus Factor) was observed for stomach and skin infections, while the lowest ICF value was noted for diabetes and gonorrhoea (0.27).Conclusions: It was concluded that the Gujrat district is rich in useful plants that can be used in the preparation of various medicines as well as be sold on the herbal market.

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Background: Pea is the most important crop and vegetable in the world but it has low yield. There is a need to find the ways for the improvement of crop productivity. Plant growth regulators (PGRs) are extensively used in many crops for enhancement of yield and quality. This study was designed to find the influence of PGRs on growth, yield and quality of pea cultivars grown in Pakistan. Methods: Pot experiments were conducted to evaluate the effect of various plant growth regulators (IAA, NAA and GA3) on pea during 2018-19. Four cultivars of pea i.e. Meteor, Green cross, Sultan and Dollar were used in these experiments. There were 0, 100 and 200 mmol’×L-1 levels for each hormone that were applied as foliar spray after 14 days of germination. Experiment was laid down in completely randomized design (CRD) with three replicates. Results: Morphological, biochemical and yield attributes significantly increased in four cultivars of pea except variety Sultan for few parameters. Cultivar Meteor showed highly positive increasing effects on growth and yield attributes as compared to other treatments. Catalases (CAT) activities were increased and peroxidase dismutase (POD) activities were reduced. It can be concluded that reduction in POD activities helped to increase the defense mechanism of plants and high CAT activities caused better balance in plant metabolism as a result growth, yield and quality of pea were increased. All the variables including shoot length, shoot biomass, number of fruits, total seed yield, carbohydrates and protein had positive correlation with phytohormones. These outcomes might be good indicators to predict the best pea cultivars that can have positive promising response for growth, yield and quality traits under plant growth regulators (PGR).

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Summary Introduction: Medicinal plants are found throughout the world but most are considered weeds. They are – directly or indirectly – the major source of medicines in pharmaceutical and herbal industries. Formulations used to prepare medicines or the method of use for these plants are mainly based on folk or traditional knowledge. This folk knowledge is not documented in many areas and needs to be explored. Objectives: This study was aimed to enlist the seasonal weed species with traditional medicinal usage in Toba Tek Singh District, Punjab, Pakistan. Methods: Field surveys were arranged in winter and summer 2019–2020 to enlist the important medicinal weeds and traditional knowledge of the local community. Data collected were as follows: local name of weed, medicinal use, method and part used. Results: Numerous wild perennial, biennial and annual plants were identified, 30 of them were ethnomedicinally important in the local community. They were grouped in 16 families. It was found that whole weed is used in many prescriptions (37%). Achyranthes aspera L. (Amaranthaceae) was the most common weed used in treating fevers, respiratory problems and asthma. Cichorium intybus L. (Asteraceae) was used in summer drinks to reduce thirst, improve digestion and liver function. Chenopodium album L. (Amaranthaceae) was used with 0.71 UV and 0.147 RFC values. Medicago polymorpha L. (Fabaceae) was used to treat kidney, intestinal and bladder infections. Its UV was 0.65 and RFC was 0.121. Tribulus terrestris L. (Zygophyllaceae) was used in impotency treatment, and in the removal of kidney stones and urinary tract infections treatment. It has 0.63 UV and 0.21 RFC values. This weed also showed the highest Fidelity Level (FL) (77%), as compared to other weeds. Conclusion: It was concluded that there are many significant medicinal weeds in the Toba Tek Singh District, Punjab, Pakistan that are used in traditional medicines in treating various disorders. These plants also showed herbal or pharmacological importance that can be used to develop medicine at commercial scale.