Srikaya leaf (Annona squamosa L.) is a plant that contains several active compounds including flavonoids, saponins, alkaloids and tannins which have antibacterial properties. Transparent antiseptic extract of srikaya leaf (Annona squamosa L.) has antibacterial activity against Staphylococcus aureus. This research method is an experimental study with physical and chemical evaluation of the preparation as well as testing of antibacterial activity using the paper disc technique diffusion method at concentrations of 1%, 2%, 3%, negative control and positive control. The results of the study at concentrations of 1%, 2% and 3% met the physical and chemical requirements as well as the antibacterial activity test with a concentration of 1% inhibition zone 15.6 mm (Strong), 2% inhibition zone 19.6 mm (Strong) and 3% zone of inhibition 21.2 mm (Very strong). It was concluded that for a concentration of 3% with an inhibition zone diameter of 21.2 mm (Very strong) the most effective.
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