Background: Laser therapy, along with the use of fluoridated compounds is a novel technique suggested for caries prevention. Casein phosphopeptide amorphous calcium phosphate (CPP-ACP) is another product suggested for this purpose. This study compared the effect of CPP-ACP and fluoride with/without Erbium, chromium-doped yttrium, scandium, gallium, and garnet laser irradiation on enamel microhardness of permanent teeth. Materials and Methods: This in vitro experimental study evaluated 35 extracted third molars. The teeth were decoronated, and the crowns were split into buccal and lingual halves. The samples were randomly divided into seven groups ( n = 10) of GC Tooth Mousse, MI Paste Plus, laser, fluoride varnish, laser + GC Tooth Mousse, laser + MI Paste Plus, and laser + fluoride varnish. The baseline microhardness was measured before the intervention. After the intervention, the samples were kept in artificial saliva for 1 h and were then immersed in the demineralizing solution for 3 h followed by 21 h of immersion in the remineralizing solution for a total period of 12 days. Finally, the teeth were kept in the remineralizing solution for 2 more days. The secondary microhardness of the teeth was then measured. Data were analyzed using the Shapiro–Wilk test, two-way ANOVA, and Tukey's Honestly Significant Difference test. Results: The fluoride varnish (14.31%) and laser + fluoride varnish (18.79%) groups experienced minimum reduction in microhardness, while the GC Tooth Mousse group experienced maximum reduction in microhardness (91.64%) ( P < 0.001). Laser irradiation before the application of remineralizing agents increased the microhardness only in laser + GC Tooth Mousse group ( P < 0.001). Conclusion: Fluoride varnish increased the enamel microhardness, while GC Tooth Mousse had no such effect. Laser therapy before the application of remineralizing agents did not significantly enhance enamel resistance to demineralization.
Background and Aim: Fibrous dysplasia (FD) and ossifying fibroma (OF) are the most important fibro-osseous lesions (FOLs) of the jaws with similarities in radiographic and morphological features while showing completely different biological behavior. Limited studies have been evaluated immunohistochemistry markers, such as osteopontin (OPN) and osteocalcin to help differentiate these two lesions. This study aimed to assess the immunoexpression of OPN and Ki67 as potential markers for differentiation of different FOLs. Materials and Methods: This cross-sectional study was conducted on 12 FD, 19 OF, and eight FOL samples retrieved from the archives of the department of oral pathology. The specimens were examined immunohistochemically using streptavidin-biotin method for OPN and Ki67. The intensity score (IS), proportional score (PS) and total score (TS) were assessed in hard and soft tissue matrix and in mesenchymal cells for Ki67. The data were analyzed by independent samples Kruskal-Wallis. Results: Osteopontin showed positive immunoreaction in both stromal and trabecular components of all FDs and OFs. Among the scores, PS and TS of bone trabeculae were significantly different in FD and OF (P=0.005). Nevertheless, no significant difference was observed in Ki67 expression in mesenchymal cells (P=0.880) and OPN scores in soft tissue matrix between the lesions; their P-value were 0.336, 0.340 and 0.415 for IS, TS, and PS, respectively. Conclusion: Osteopontin can serve as a useful marker for differential diagnosis of FD and OF. However, we suggest evaluation of other NCMPs, especially functionally similar molecules such as bone sialoproteins (BSPs) in FOLs for differential diagnosis.
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