Objective: To evaluate the level of contamination in different hospital's wards and kitchens in AL-Yarmouk teaching hospital in Baghdad -Iraq. Method:The study was done from the 1 st of January, 2017 to the 30 th of June, 2019. A total of 7742sterile cotton swabs were collected randomly from the following wards: Burns, RCU, CCU, NICU, (Dialysis, Bronchoscopy, Endoscopy) unites and Kitchens. Samples were cultured in different agars and incubated for 24-48 hrs at 25 °C and 37 °C for fungi and bacterial growth, respectively.Results: Out of 7742 sample, 1227 swabs were reported positive. By 2017, Gram-positive bacteria were more prevalent with 59.5%, and Bacillus was the most common isolated .The rates of Gram-negative bacteria became more predominant with 54.9% and 69.4% in 2018 and 2019, respectively and E. coli was the main isolated bacteria with prevalence rates of 32.1% in (2018) and 42.4% in (2019). The most contaminated wards were

Background:The hospital environment, the design of buildings especially operating theaters and associated facilities play an important role in the spread of nosocomial infections and surgical site infection.Methods: A descriptive study. It covers 30 months; Weekly swabs were collected from thirty-two operating theaters. Results:The highest rate of contamination was in the general surgical OT with 12.9%, followed by Orthopedic OT (10.7%). On the other hand, Ophthalmic OT recorded the least bacterial contamination with (3.1%) only.During the three years' time of the study; we saw heavy growth of G-ve bacteria:P.aurogenosa195(35.8%), 137(43.4%) and 48(53.3%), thenE. coli141(25.9%), 115(%) and 28(%) respectively. Conclusion:Contamination was identified in all OT in different branches. Bacterial growth reported throughout the year and in different months. Despite the availability and usage of the sterilization materials, contamination still there.