Objective: The present study deals with the cytotoxic activity of n-hexane and ethyl acetate extracts of Calotropis gigantea L. stem bark and its fractions such as A, B, C, D and E fractions on murine leukemia cell line P388. Methods:The crude extracts of C. gigantea stem bark were prepared using n-hexane and ethyl acetate solvents. The plant extracts were subjected to vacuum liquid chromatography followed by TLC. According to the similarity of stain patterns, the fractions were combined. The extracts and its combined fractions were then subjected for the phytochemical test. Cytotoxic activity of those extracts and its combined fractions were tested using MTT assay. Fraction D was subjected to gravity column chromatography followed by TLC. Then, fractions A, B, and D2 were crystallized and subjected to GC-MS. Results:The qualitative screening of n-hexane extract of Calotropis gigantea L. stem bark for secondary metabolites showed the presence of terpenoid, flavonoids, phenolics and coumarins. While the ethyl acetate extract contained phenolics, steroids, flavonoids, saponins and coumarins compounds. IC50 values for n-hexane extract and E fraction are 76.29 µg/ml and 18.48 µg/ml, respectively. In the ethyl acetate extract and C fraction obtained IC50 Conclusion:Cytotoxic activity from E fraction of n-hexane extract of C. gigantea stem bark is the most potent and containing flavonoids, phenolics and coumarins. The main components from several compounds of n-hexane extract of C. gigantea are germacrane-A, (-)-globulol, urs-12-ene and veridiflorol.values 57.05 µg/ml and 52.58 µg/ml.
A new method for determining ploidy levels in cytochimeral plants was developed by examining the maximum number of nucleoli per cell . Colchicine-treated plants of Allium wakegi Araki and A. fistulosum L., which have different ploidy levels in the first (LI), the second (LII), and the third (LIII) germ layer, were used together with untreated 2-2-2 plants of the same species . Nucleoli in guard cells for LI and in mesophyll cells for LII were stained in a 50% AgNO3 aqueous solution at 55° C for three hours under dark, humid conditions . In both species the ploidy levels of LI determined by the maximum number of nucleoli per guard cell accorded well with those determined by guard cell length. In A . fistulosum the ploidy levels of LII determined by the maximum number of nucleoli per mesophyll cell clearly agreed with those determined by pollen size. This method provided more precise and efficient identification for LI and LII ploidy than the conventional methods of using guard cell length for LI and pollen size for LII .
Drought is one of the important climate changes that could affect the early planting of cassava to produce a high yield. Lampung province having a specific climate, wet tropical dry-season is the center of cassava production in Indonesia. Consequently, the objective was to evaluate the effect of micro fertilizers on the growth and yield of two cassava clones planting in the early dry season. This research was conducted on Ultisol soil of Tanjung Bintang District, South Lampung from May 2019 to April 2020. Treatment was arranged by factorial (2x2) in a Randomized Complete Block Design (RCBD) which 3 replications. The first factor was two applications of micro fertilizer, 0 and 40 kg ha−1. The second factor was two cassava clones, UJ-5 and BW-1. Results showed that the leaf number of UJ-5 at 6 MAP and 9 MAP was increased by micro fertilizer. Yet, the leaf number of BW-1 at 6 MAP and 9 MAP was not increased by micro fertilizer. It seems that UJ-5 could be more tolerant to drought in early planting time. Consequently, the tuber fresh weight of UJ-5 was heavier due to micro fertilizer but lower starch content compared to BW-1. It could be concluded that UJ-5 when planted at early dry-season would show higher tuber fresh weight with lower starch content than BW-1.
This research aims to obtain the proper nutrient concentration and the best nutrient concentration and increase the growth and yield of pakcoy through the floating hydroponic system. The research was conducted in the Experimental Farm of the Faculty of Agriculture, University of Riau from October to November 2019. The research used a non-factorial completely randomized design (CRD), consisting of 5 treatments and 4 replications. The given treatment was the nutrient concentration of AB Mix consisting of 5 levels: AB Mix 1000 ppm, AB Mix 1200 ppm, AB Mix 1400 ppm, AB Mix 1600 ppm, and AB Mix 1800 ppm. The parameters observed were plant height, leaf number, leaf area, root volume, plant fresh weight, and worthy weight consumption. The result showed that all the given treatments did not have significant towards all parameters. The concentration of 1000 ppm is the best concentration of some else, because more efficient, economic and saves the solution uses of nutrien in concentration. Based on the results of a study that has been conducted, to gain growth and the results of white pakcoy plant are both hydroponic can usea concentration of AB Mix 1000 ppm.
The important part of cassava root is starch which is probably controlled by starch synthase type IV (SSIV) gene. The information of micro nutrient and harvest age related to the activity starch synthase type IV (SSIV) gene is still very rare. The objectives of this study were to evaluate root fresh weight of cassava, to compare yield of storage root, and to evaluate the activity of starch synthase type IV (SSIV) gene by real-time quantitative polymerase chain reaction (RT-PCR) applied by micro nutrient fertilizer. Treatments were arranged by factorial (3 × 3) in randomized complete block design (RCBD) with three replications used as block. The first factor was three different dosages of micro nutrient fertilizer as 0, 20, and 40 kg/ha. The second factor was harvest ages as 7, 8, and 10 months after planting (MAP). The micro nutrient fertilizer mainly contents of 5,888 ppm Fe and 1,368 ppm Zn. Variables were leaf number (LN), leaf fresh weight (LFW), leaf dry weight (LDW), stem fresh weight (SFW), stem dry weight (SDW), root fresh weight (RFW), root dry weight (RDW), skin root fresh weight (SRFW), skin root dry weight (SRDW), starch content, and activity of SSIV gene. The result showed that RDW of cassava applied by 40 kg micro nutrient/ha was significantly increased at 10 MAP. The increase in RDW was due to mainly high SSIV gene activity. Additionally, the SSIV gene activity caused by 20 kg micro nutrient/ha treatment showed almost as twice as those by 40 kg micro nutrient/ha. Keywords: Real-time PCR, Root dry weight, Root yield, Starch, Stem dry weight
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