In Mexico, the accelerated and unplanned growth of population causes the increment in informal facilities, complicating access to essential services such as adequate sanitation and organic waste management, where the most affected are usually the vulnerable population. The Mexican government has implemented programs and support to encourage the use of ecotechnologies and thus provide alternatives to improve their living conditions by taking advantage of local resources and waste. However, these programs and supports lack planning, creating little interest from users. This research proposes an acceptance strategy for the integration of ecotechnologies that adapt to the lifestyle of populations that need an alternative for their method of sanitation and organic waste management. The strategy is mainly based on a course of action that operates with four main lines of intervention. It was executed as a practical exercise with biodigesters, in the Mexican community of La Trinidad, in the municipality of Tequisquiapan, Querétaro. With the help of the strategy, important factors for the end-user were identified that must be considered when integrating one ecotechnology into the population. In general terms, the strategy showed an improvement in sanitation and organic waste management and a greater acceptance of biodigesters as an alternative of use.
Currently, agricultural systems are inadequate to meet the demand of the population, coupled with the constant degradation of natural resources. Therefore, it is necessary to explore alternatives to increase the productivity and quality of crops with minimal environmental impact. The use of plant growth-promoting bacteria can provide solutions to some agri-environmental problems and replace or minimize conventional agricultural practices. In this study, a Bacillus pumilus strain with plant growth-promoting properties was isolated from mature compost. In vitro, the ability of Bacillus pumilus to solubilize phosphate, inhibit the growth of phytopathogenic fungi, and its effect on the germination of tomato and lettuce seeds was evaluated. In vivo, its effect on stem thickness, height, and the number of leaves of tomato and lettuce seedlings was studied. The results show that, in vitro, Bacillus pumilus solubilizes phosphate, inhibits the growth of the fungus Fusarium oxysporum, and increases the germination percentage of tomato seeds. The results, in vivo, demonstrate that the bacteria increases the stem thickness of tomato seedlings, while, in lettuce, it increases the stem thickness and the number of leaves. The outcome implies that Bacillus pumilus has properties as a plant growth promoter and can be used as a promising inoculant to enhance the growth of tomato and lettuce seedlings.
The technology for reproducing orchids in vitro has had to evolve due to the demand for these plants and the high cost of the biotechnology used due to the agar, the gelling agent. Consequently, research has tended to search for natural substitutes for agar. Our work describes the use of pectin and mucilage hydrocolloids extracted from the local waste matter of two species of Opuntia (O. ficus-indica and O. robusta) to study as a gelling agent in vitro culture media for Cattleya sp. These hydrocolloids were obtained by alkaline hydrolysis. Subsequently, these were used in proportions of 0.8%, 0.6%, 0.4%, and 0.2% in combination with agar to study the gelation time, texture profile analysis (TPA), seed germination under light and dark conditions, and a phenological study, including orchid analyses of leaves and roots, root and leaf length, seedling height, and width of the best-designed treatment were studied. Our results demonstrate that the treatment composed of 0.4% O. ficus-indica pectin and 0.4% agar improves the germination time, plant growth, and the number of leaves and roots, resulting in a biostimulant formula for optimal in vitro growth of Cattleya sp.
In Vitro culture is a technique commonly used for plant research. Nevertheless, it is more expensive than traditional methods of production, due to the use of the culture medium gelling agent called agar. Recent studies have been searching for alternative substances in raw materials with the same characteristics but which can be extracted easier than agar. The dietary fiber of the nopal cactus (Opuntia) is a rich source of hydrocolloids (pectin and mucilage). These hydrocolloids have the ability to gel in combination with the indicated solution. In this chapter, we will focus on the study of the hydrocolloids from nopal cactus to replace agar partially and/or totally as a gelling agent using in vitro culture media benefiting from the molecular structure and mechanical properties of the compounds.
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