Ojek online is one of the on-trend informal jobs in Medan. Yet, an ojek online driver can face accidents and illnesses due to irregular times when doing this job. One of the complaints of the ojek online drivers is fatigue. The manifestation of fatigue is characterized by an increase of blood lactic acid concentration. The blood lactic acid is in concentration threshold of 4 mmol/l blood which indicates that muscle activity has become anaerobic (without oxygen supply). If the blood lactic acid concentration threshold reaches 8 mmol/l blood or more, it indicates that a person has begun to experience physical fatigue. This study was a quantitative research and it involved 40 drivers. The independent variables were ages, durations of work, working periods and workloads. Meanwhile, the fatigue was as the dependent variable. The result of research showed that majority of ojek online drivers experienced fatigue with an average lactic acid level of 2.3 mmol/l. The majority of ojek online drivers had worked for 12 months with an average working of 12 hours/day. The ojek online drivers' workload reached 150 Km/day. It showed that there were effects of ages (0.045) and workloads (0.000) on the fatigue of ojek online drivers.
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