SummaryA semi-automated imaging system is described to quantitate estrogen and progesterone receptor immunoreactivity in human breast cancer. The system works for any conventional method of image acquisition using microscopic slides that have been processed for immunohistochemical analysis of the estrogen receptor and progesterone receptor. Estrogen receptor and progesterone receptor immunohistochemical staining produce colorimetric differences in nuclear staining that conventionally have been interpreted manually by pathologists and expressed as percentage of positive tumoral nuclei. The estrogen receptor and progesterone receptor status of human breast cancer represent important prognostic and predictive markers of human breast cancer that dictate therapeutic decisions but their subjective interpretation result in interobserver, intraobserver and fatigue variability. Subjective measurements are traditionally limited to a determination of percentage of tumoral nuclei that show positive immunoreactivity. To address these limitations, imaging algorithms utilizing both colorimetric (RGB) as well as intensity (gray scale) determinations were used to analyze pixels of the acquired image. Image acquisition utilized either scanner or microscope with attached digital or analogue camera capable of producing images with a resolution of 20 pixels /10 µ. Areas of each image were screened and the area of interest richest in tumour cells manually selected for image processing. Images were processed initially by JPG conversion of SVS scanned virtual slides or direct JPG photomicrograph capture. Following image acquisition, Correspondence to: Sanford H. Barsky. Tel: 614-292-4692; fax: 614-688-5632; e-mail: sanford.barsky@osumc.edu images were screened for quality, enhanced and processed. The algorithm-based values for estrogen receptor and progesterone receptor percentage nuclear positivity both strongly correlated with the subjective measurements (intraclass correlation: 0.77; 95% confidence interval: 0.59, 0.95) yet exhibited no interobserver, intraobserver or fatigue variability. In addition the algorithms provided measurements of nuclear estrogen receptor and progesterone receptor staining intensity (mean, mode and median staining intensity of positive staining nuclei), parameters that subjective review could not assess. Other semi-automated image analysis systems have been used to measure estrogen receptor and progesterone receptor immunoreactivity but these either have required proprietary hardware or have been based on luminosity differences alone. By contrast our algorithms were independent of proprietary hardware and were based on not just luminosity and colour but also many other imaging features including epithelial pattern recognition and nuclear morphology. These features provide a more accurate, versatile and robust imaging analysis platform that can be fully automated in the near future. Because of all these properties, our semi-automated imaging system 'adds value' as a means of measuring these important nuclea...
Background: Immunocytochemical methods for quantitating Her-2/neu immunoreactivity rest on subjective semi-quantitative interpretations with resulting interobserver, intraobserver, and fatigue variability. Methods: To standardize and quantitate measurements of Her-2/neu immunoreactivity, we created epithelial-recognition and specific membrane-recognition algorithms, which could image breast cancer cells against a background of stroma, compartmentalize the cancer cell into nucleus, cytoplasm and membrane, and quantitate the degree of Her-2/neu membrane immunoreactivity based on both gray scale intensity and RGB colorimetric determinations. Image acquisition utilized either scanner or microscope with attached camera with a resolution of 20 pixels/10 lm. Areas of 150 whole slides were screened and the regions of interest manually selected for image processing. Three hundred TMA cores were directly processed. Images were acquired by jpg conversion of svs virtual slides or direct jpg photomicrograph capture. Images were then assessed for quality and processed. Results: The digital algorithms successfully created a semi-automated imaging system whose algorithm-based ordinal values for Her-2/neu both strongly correlated with the subjective measurements (intraclass correlation: 0.84;
The objective of this research is to develop to the proof-of-concept stage, a fault tolerant diagnosis system for the RADARSAT-1 attitude control system (ACS) telemetry. The proposed system is using computational intelligence (CI) to detect and isolate faults and also to infer cause of failures from the telemetry data time series history using functional models of satellite ACS. The proposed work is based on a distributed nonlinear, self-learning and self-adapting models (that can learn and improve themselves overtime) adjusting to the environment and constraints to which the real data is subjected. The key research and development issue is to create prototype models that will be able to integrate telemetry data and address the fault diagnosis problem without human intervention and expertise. The proposed work aims to support space industries' future interests in on-board fault diagnosis for next generation spacecraft by utilizing CI techniques as well as to help ground system operators in performing calibrations, anomaly detection, isolation and recovery, or testing of components.
In cloud computing, load balancing among the resources is required to schedule a task, which is a key challenge. This paper proposes a dynamic degree memory balanced allocation (D2MBA) algorithm which allocate virtual machine (VM) to a best suitable host, based on availability of random-access memory (RAM) and microprocessor without interlocked pipelined stages (MIPS) of host and allocate task to a best suitable VM by considering balanced condition of VM. The proposed D2MBA algorithm has been simulated using a simulation tool CloudSim by varying number of tasks and keeping number of VMs constant and vice versa. The D2MBA algorithm is compared with the other load balancing algorithms viz. Round Robin (RR) and dynamic degree balance with central processing unit (CPU) based (D2B_CPU based) with respect to performance parameters such as execution cost, degree of imbalance and makespan time. It is found that the D2MBA algorithm has a large reduction in the performance parameters such as execution cost, degree of imbalance and makespan time as compared with RR and D2B CPU based algorithms
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