Ice growth mitigation is a pervasive challenge for multiple
industries.
In nature, ice-binding proteins (IBPs) demonstrate potent ice growth
prevention through ice recrystallization inhibition (IRI). However,
IBPs are expensive, difficult to produce in large quantities, and
exhibit minimal resilience to nonphysiological environmental stressors,
such as pH. For these reasons, researchers have turned to bioinspired
polymeric materials that mimic IBP behavior. To date, however, no
synthetic polymer has rivaled the ability of native IBPs to display
IRI activity at ultralow nanomolar concentrations. In this work, we
study the IRI activity of peptides and polypeptides inspired by common
ice-binding residues of IBPs to inform the synthesis and characterization
of a potent bioinspired polymer that mimics IBP behavior. We show
first that the threonine polypeptide (pThr) displays
the best IRI activity in phosphate-buffered saline (PBS). Second,
we use pThr as a molecular model to synthesize and
test a bioinspired polymer, poly(2-hydroxypropyl methacrylamide) (pHPMA). We show that pHPMA exhibits potent
IRI activity in neutral PBS at ultralow concentrations (0.01 mg/mL). pHPMA demonstrates potent IRI activity at low molecular
weights (2.3 kDa), with improved activity at higher molecular weights
(32.8 kDa). These results substantiate that pHPMA is a robust molecule that mitigates ice crystal growth at concentrations
similar to native IBPs.
Ice growth mitigation is a pervasive challenge for multiple industries. In nature, ice-binding proteins (IBPs) demonstrate potent ice-growth prevention through ice recrystallization inhibition (IRI). However, IBPs are expensive, difficult to produce in large quantities, and exhibit minimal resilience to non-physiological environmental stressors, such as elevated pH. For these reasons, researchers have turned to polymeric bioinspired mimics. To-date, however, no mimic has rivaled the ability of native IBPs to display IRI activity at ultra-low nanomolar concentrations. In this work, we study the IRI activity of peptides and polypeptides inspired by common ice-binding residues of IBPs to inform the synthesis and characterization of a potent bioinspired polymer mimic. We show first that the threonine polypeptide (pThr) displays the best IRI activity in phosphate-buffered saline (PBS). Second, we use pThr as a molecular model to synthesize and test a new bioinspired polymer mimic, poly(2-hydroxypropyl methacrylamide) (pHPMA). We show that pHPMA exhibits potent IRI activity in neutral PBS at nanomolar concentrations. These results substantiate that pHPMA outperforms poly(vinyl alcohol) (PVA), the current top performing IBP mimic in the field, in terms of effectiveness at mitigating ice crystal growth at concentrations akin to native IBPs.
Ice growth mitigation is a pervasive challenge for multiple industries. In nature, ice-binding proteins (IBPs) demonstrate potent ice-growth prevention through ice recrystallization inhibition (IRI). However, IBPs are expensive, difficult to produce in large quantities, and exhibit minimal resilience to non-physiological environmental stressors, such as elevated pH. For these reasons, researchers have turned to polymeric bioinspired mimics. To-date, however, no mimic has rivaled the ability of native IBPs to display IRI activity at ultra-low nanomolar concentrations. In this work, we study the IRI activity of peptides and polypeptides inspired by common ice-binding residues of IBPs to inform the synthesis and characterization of a potent bioinspired polymer mimic. We show first that the threonine polypeptide (pThr) displays the best IRI activity in 2 phosphate-buffered saline (PBS). Second, we use pThr as a molecular model to synthesize and test a new bioinspired polymer mimic, poly(2-hydroxypropyl methacrylamide) (pHPMA). We show that pHPMA exhibits potent IRI activity in neutral PBS at nanomolar concentrations. These results substantiate that pHPMA outperforms poly(vinyl alcohol) (PVA), the current top performing IBP mimic in the field, in terms of effectiveness at mitigating ice crystal growth at concentrations akin to native IBPs.
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