Four bacterial strains were isolated from Khor Al-Zubair channel, southern Iraq based on a high growth rate on crude oil and on hydrocarbon degradation ability. The strains were preliminarily identified based on morphological observation, and by the Vitek II system. The isolates were identified as Brevundimonas diminuta /vesicularis, Vibrio vulnificus, Sphingomonas paucimobilis and Ochrobactrum anthropic. However, the ability of these strains to utilize crude oil (0.25%; 0.5%; 1% and 2% v/v) varied both in rates of utilization and in growth profiles. The components of crude oil degraded by the isolates were determined by capillary gas chromatography. Sphingomonas paucimobilis and Vibrio vulnificus were the most effective bacteria degrade PAH on seventh day incubation, especially in concentration 2.0 mg/l (97.39% and 84.23%) respectively, meanwhile, Brevundimonas diminuta/ vesicularis was highly effective degrade PAH (73.41% a nd 62.08%) in concentration 1.0 and 0.5 mg/l, respectively. On the other hand, Ochrobactrum anthropi was the lowest in all concentrations.
Methylotrophs bacteria are ubiquitous, and they have the ability to consume single carbon (C1) which makes them biological conversion machines. It is the first study to find facultative methylotrophic bacteria in contaminated soils in Iraq. Conventional PCR was employed to amplify MxaF that encodes methanol dehydrogenase enzyme. DNA templates were extracted from bacteria isolated from five contaminated sites in Basra. The gene specific PCR detected Methylorubrum extorquens as the most dominant species in these environments. The ability of M. extorquens to degrade aliphatic hydrocarbons compound was tested at the laboratory. Within 7 days, gas chromatographic (GC) studies of remaining utilized crude oil revealed that 61.14 % of the initial content had been degraded, and GC fingerprinting of the utilized aliphatic compounds revealed significant reductions in C12, C13, C14, and C15. Globally this is the first time found a new strain of M. extorquens has the ability to degrade aliphatic hydrocarbons compound. Conventional PCR and gene sequencing revealed the presence of the facilitative methylotrophic bacteria in polluted areas in Basra. M. extorquens was dominant and showed a substantial ability to degrade crude oil which makes them an important tool to be employed in bioremediation.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.