In an effort to study plants of the Rubiaceae from Panama, 299 herbarium samples, representing 133 species, were subjected to alkaloid screening. From this total, 234 samples (78%) afforded a positive test for alkaloids, and 99 samples (33%) gave a positive reaction for indole alkaloids; 109 species (82%) contain alkaloids and 58 (44%) produce indole alkaloids. This is the first time that 102 (94%) and 52 (90%) species are reported as alkaloid and indole alkaloid bearing, respectively. The results of 10 out of 15 species, that is 67%, corroborate earlier studies. The richest indole alkaloid species were: Materials and Methods Plant materialLeaf, flower, fruit or seed samples were provided by Mireya Correa, Director of the Herbarium of the University of Panama (PMA). Screening of alkaloidsLess than 1 g of samples was moistened with diluted 10% ammonia, shaken with chloroform for 24, and brought to acid-base extraction to yield the alkaloid extracts. The extracts were redissolved in 1 ml of chloroform, and 15 drops of the extract were spotted on a filter paper. For comparison, 5, 10 and 15 drops of 0.5 mg/ml emetine hydrochloride were spotted on the same filter paper. Then, the paper was sprayed with Dragendorff 's reagent. A positive test for alkaloids was recorded when an orange colour ranging from light (+) to very deep (++++) developed. A score of four crosses (++++) means that the color reaction of the sample is more intense
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