Abstract:Since 2003, non-compliant aflatoxin concentrations have been detected in maize produced in Italy. The most successful worldwide experiments in aflatoxin prevention resulted from distribution of atoxigenic strains of Aspergillus flavus to displace aflatoxin-producers during crop development. The displacement results in lower aflatoxin concentrations in harvested grain. The current study evaluated in field performances of two atoxigenic strains of A. flavus endemic to Italy in artificially inoculated maize ears and in naturally contaminated maize. Co-inoculation of atoxigenic strains with aflatoxin producers resulted in highly significant reductions in aflatoxin concentrations (>90%) in both years only with atoxigenic strain A2085. The average percent reduction in aflatoxin B 1 concentration in naturally contaminated maize fields was 92.3%, without significant differences in fumonisins between treated and control maize. The vegetative compatibility group of A2085 was the most frequently recovered A. flavus in both treated and control plots (average 61.9% and 53.5% of the A. flavus, respectively). A2085 was therefore selected as an active ingredient for biocontrol products and deposited under provisions of the Budapest Treaty in the Belgian Co-Ordinated Collections of Micro-Organisms (BCCM/MUCL) collection (accession MUCL54911). Further work on development of A2085 as a tool for preventing aflatoxin contamination in maize produced in Italy is ongoing with the commercial product named AF-X1™.Keywords: biopesticide; biocontrol; mycotoxin; fumonisin; maize; VCG; SSR; AF-X1™; aflatoxin prevention; Europe Key Contribution: The first active ingredient for biological control of aflatoxins in maize produced in Europe was selected. Commercial field trials in Italy support further development and registration of the biopesticide named AF-X1™.
Among plant fungal diseases, those affecting cereals represent a huge problem in terms of food security and safety. Cereals, such as maize and wheat, are very often targets of mycotoxigenic fungi. The limited availability of chemical plant protection products and physical methods to control mycotoxigenic fungi and to reduce food and feed mycotoxin contamination fosters alternative approaches, such as the use of beneficial fungi as an active ingredient of biological control products. Competitive interactions, including both exploitation and interference competition, between pathogenic and beneficial fungi, are generally recognized as mechanisms to control plant pathogens populations and to manage plant diseases. In the present review, two examples concerning the use of competitive beneficial filamentous fungi for the management of cereal diseases are discussed. The authors retrace the history of the well-established use of non-aflatoxigenic isolates of Aspergillus flavus to prevent aflatoxin contamination in maize and give an overview of the potential use of competitive beneficial filamentous fungi to manage Fusarium Head Blight on wheat and mitigate fusaria toxin contamination. Although important steps have been made towards the development of microorganisms as active ingredients of plant protection products, a reasoned revision of the registration rules is needed to significantly reduce the chemical based plant protection products in agriculture.
Fabrics manufactured in Italy with 100% Sardinian sheep breed wool, traditionally named ‘Orbace’, and with local Tuscan wool from 50% Sardinian and 50% Comisana sheep breeds wool (Tuscan Cloth), were subjected to sensory evaluation, through descriptive analysis performed by a trained panel, and to an instrumental evaluation method, through FAST – Fabric Assurance by Simple Testing. The correlation between instrumental data and sensory attributes was investigated. Sensory evaluation indicated that Orbace fabrics, originated from 100% Sardinian wool, had higher grittiness, stiffness, force of compression and hand friction and lower softness and tensile stretch, as compared to the fabrics obtained from mixed wool. Principal component analysis (PCA) indicated a high correlation for grittiness, stiffness and force of compression in one direction, tensile stretch, softness, warmth and fullness in the opposite, clearly discriminating in this dimension Orbaces from Tuscan fabrics. This discrimination appears to be an effect of the wool used for the different fabrics. Differences in key sensory traits were also recorded among fabrics derived from the same raw material, but differing in weave. Thickness, as the key attribute of the second PCA dimension, provided discriminations within groups and is reasonably perceived as an effect of different weaves. Canonical Correlation Analysis indicated that measurements performed with FAST were correlated with a number of basic touch traits, including softness, stiffness, force of compression and tensile stretch. Orbace and Tuscan Cloth handle definition, on the basis of sensory and instrumental measures, will be of use to describe and protect original products.
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