A lactate amperometric biosensor based on lactate oxidase immobilized in a poly(o-phenylenediamine) lilm grown on a polypyrrole-modified platinum electrode is described. The biosensor, which is fabricated by an all-electrochemical procedure, displays a response time in the low second range and an interferent rejection dciency never achieved before. Ascorbate, urate, and cysteine, which are the most common endogenous electroactive components, produce a nondetectable bias even at their maximum physiological concentrations in blood. Exogenous paracetamol, which at present is the most diliicult to manage electroactive interferent, is also very efficiently excluded; at the maximum observable physiological level of 0.2 mM, a truly negligible lactate bias of only 3 pM (Le., 0.15% at 2 mM lactate) is produced.

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In this work, for the first time, snail slime from garden snails “Helix Aspersa Müller”, has been used to induce the formation of eco-friendly gold nanoparticles (AuNPs-SS), suitable for biomedical...

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