We examined the effects of exercise intensity and a 10-wk cycle ergometer training program [5 days/wk, 1 h, 75% peak oxygen consumption (VO2 peak)] on plasma free fatty acid (FFA) flux, total fat oxidation, and whole body lipolysis in healthy male subjects (n = 10; age = 25.6 +/- 1.0 yr). Two pretraining trials (45 and 65% of VO2 peak) and two posttraining trials (same absolute workload, 65% of old VO2 peak; and same relative workload, 65% of new VO2 peak) were performed by using an infusion of [1-13C]palmitate and [1,1,2,3, 3-2H]glycerol. An additional nine subjects (age 25.4 +/- 0.8 yr) were treated similarly but were infused with [1,1,2,3,3-2H]glycerol and not [1-13C]palmitate. Subjects were studied postabsorptive for 90 min of rest and 1 h of cycling exercise. After training, subjects increased VO2 peak by 9.4 +/- 1.4%. Pretraining, plasma FFA kinetics were inversely related to exercise intensity with rates of appearance (Ra) and disappearance (Rd) being significantly higher at 45 than at 65% VO2 peak (Ra: 8.14 +/- 1.28 vs. 6.64 +/- 0.46, Rd: 8. 03 +/- 1.28 vs. 6.42 +/- 0.41 mol. kg-1. min-1) (P

Ice-climbers frequently use the squeezing of rubber rings for increasing their isometric strength-endurance in the forearm muscles. The aim of this study was to ascertain whether such training influences oxygenation and endurance of forearm muscles at higher as well as lower testing intensities. Fourteen healthy young ice-climbers were divided and randomized into two groups. Group A performed a specific ice-climbing test, an ice-axe-grasping (axe weight 750 g) until fatigue. Group B performed 150 N isometric hand-squeezing of dynamometer until fatigue. Both groups performed similar training of squeezing a rubber ring at 30% of Maximal Voluntary Contraction (MVC) for 6 weeks. The forearm oxygenation was assessed by relative saturation of oxygen (RSO(2)), total hemoglobin concentration (RTOTHb), the concentration of oxygenated hemoglobin (ROXYHb) and concentration of deoxygenated hemoglobin (RDEOXYHb). The results revealed that muscle strength-endurance training increased performance of forearm muscles during 150 N contraction with an accompanied increase in oxygenation of the exercising muscles. In contrast, the same training did not influence the performance of forearm muscles during ice-axe-grasping in spite of increased oxygenation. Muscle oxygenation during intense isometric contraction is low in spite of an increase observed in training. This may be due to oxygenation levels that were below the limit where oxygenation may influence the duration of the contraction. Increased oxygenation may have occurred due to an increased blood flow and perfusion through superficial muscles or layers may not have contributed to the generation of the force of the contraction, as would be the case in deeper muscle layers.

IntroductionWhile hypoxia is known to decrease peak oxygen uptake (V.⁢o2 max) and maximal power output in both adults and children its influence on submaximal exercise cardiorespiratory and, especially, muscle oxygenation responses remains unclear.MethodsEight pre-pubertal boys (age = 8 ± 2 years.; body mass (BM) = 29 ± 7 kg) and seven adult males (age = 39 ± 4 years.; BM = 80 ± 8 kg) underwent graded exercise tests in both normoxic (PiO2 = 134 ± 0.4 mmHg) and hypoxic (PiO2 = 105 ± 0.6 mmHg) condition. Continuous breath-by-breath gas exchange and near infrared spectroscopy measurements, to assess the vastus lateralis oxygenation, were performed during both tests. The gas exchange threshold (GET) and muscle oxygenation thresholds were subsequently determined for both groups in both conditions.ResultsIn both groups, hypoxia did not significantly alter either GET or the corresponding V.⁢o2 at GET. In adults, higher trueV.E levels were observed in hypoxia (45 ± 6 l/min) compared to normoxia (36 ± 6 l/min, p < 0.05) at intensities above GET. In contrast, in children both the hypoxic trueV.E and V.⁢o2 responses were significantly greater than those observed in normoxia only at intensities below GET (p < 0.01 for trueV.E and p < 0.05 for V.⁢o2). Higher exercise-related heart rate (HR) levels in hypoxia, compared to normoxia, were only noted in adults (p < 0.01). Interestingly, hypoxia per se did not influence the muscle oxygenation thresholds during exercise in neither group. However, and in contrast to adults, the children exhibited significantly higher total hemoglobin concentration during hypoxic as compared to normoxic exercise (tHb) at lower exercise intensities (30 and 60 W, p = 0.01).ConclusionThese results suggest that in adults, hypoxia augments exercise ventilation at intensities above GET and might also maintain muscle blood oxygenation via increased HR. On the other hand, children exhibit a greater change of muscle blood perfusion, oxygen uptake as well as ventilation at exercise intensities below GET.

The aim of the study was to find the influence of training on the oxygenation status of a forearm muscle during submaximal isometric contraction. Six subjects performed a test of isometric contraction of a forearm muscle by continuous pressing of a handgrip dynamometer with a force of 15 kp, until exhaustion. The continuous measurements of oxygen saturation (%) (OXY), concentration of oxygenated haemoglobin (pM) (OXYHb), deoxygenated haemoglobin (pM) (DEOXYHb) and total haemoglobin (pM) (TOTHb) in blood, which passed through the muscle, were made by the near infrared spectroscope Oxymeter 96208 (ISS, USA). This test was repeated before and after the four weeks training period, which consisted of 5 continuous isometric contractions of 30 s at the beginning and of 1:15 min during the last week. Training did not significantly influence maximal isometric force (55.5 f 10 kp before, 60.2 k 9 kp after). On the other hand, the duration of isometric contraction increased from 27 1 k 1 17 s to 388 & 152 s (P < 0.05). Subjects were substantially different in duration of muscle contraction and also in oxygenation status during the contractions. Therefore, the relationship between these parameters and the training effect was calculated. The training effect was represented as the difference between the duration of muscle contraction before and after the training period. This parameter significantly correlated with relative oxygen saturation (r = -0.88; P < 0.05) and with relative concentration of deoxygenated haemoglobin (r = 0.87; P < 0.05). These results demonstrated the importance of individual training adaptations of forearm muscles. The increase of the duration of submaximal isometric contraction,-as a training effect, can be dependent on larger muscle deoxygenation.

The effect of inspiratory muscle fatigue on acid-base status and performance during race-paced middle-distance swimming Inspiratory muscle fatigue, swimming and acid-base status

No abstract

To study how two types of testing protocols for the determination of the [LA] and blood pH kinetics during running match each other, a group of nine runners participated in two testing protocols. The first protocol consisted of a sequences of 8 x 2000 m runs at constant speed, which was increased on different testing days. The first protocol was used for the determination of running speed (v), [LA], pH and heart rate (HR) corresponding to the Lactate Threshold (LT) and the Threshold of Acidosis (TA), both occurring at similar running speeds (4.21 +/- 0.44 and 4.22 +/- 0.40 m/s) (mean +/- SD) and HR (159 +/- 7 and 160 +/- 9 1/min), [LA] = 2.0 +/- 0.6 mmol/l and pH = 7.411 +/- 0.018. Maximal steady values for [LA] (maxLAss) and minimal steady values for pH (minpHss) obtained by the second protocol were higher (p < 0.01) according to running speed (4.62 +/- 0.38 and 4.66 +/- 0.38 m/s), HR (172 +/- 7 and 174 +/- 8 1/min) and [LA] (5.7 +/- 1.3 mmol/l) and lower according to pH (7.364 +/- 0.021), respectively. Unlike similar running speeds determined by LT and TA, the minimal steady pH level occurred at a slightly higher speed than the speed at maxLAss. Additionally, we found a drift of pH towards a resting level, when [LA] fluctuated around a steady level. Furthermore, the parameters of pH kinetics correlated better with the running speed of the 4000 m run that was taken as the parameter of short endurance performance, than those of [LA] kinetics. We conclude that these differences in [LA] and pH kinetics could serve to predict the capabilities of runners with respect to the two endurance performance types: long and short.

We aimed to elucidate potential differential effects of hypoxia on cardiorespiratory responses during submaximal cycling and simulated skiing exercise between adults and pre-pubertal children. Healthy, low-altitude residents (adults, N=13, Age=40±4yrs.; children, N=13, age=8±2yrs.) were tested in normoxia (Nor: PO=134±0.4 mmHg; 940 m) and normobaric hypoxia (Hyp: PO=105±0.6 mmHg; ~3 000 m) following an overnight hypoxic acclimation (≥12-hrs). On both days, the participants underwent a graded cycling test and a simulated skiing protocol. Minute ventilation (V), oxygen uptake (VO), heart rate (HR) and capillary-oxygen saturation (SpO) were measured throughout both tests. The cycling data were interpolated for 2 relative workload levels (1 W·kg & 2 W·kg). Higher resting HR in hypoxia, compared to normoxia was only noted in children (Nor:78±17; Hyp:89±17 beats·min; p<0.05), while SpO was significantly lower in hypoxia (Nor:97±1%; Hyp:91±2%; p<0.01) with no between-group differences. The V, VO and HR responses were higher during hypoxic compared to normoxic cycling test in both groups (p<0.05). Except for greater HR during hypoxic compared to normoxic skiing in children (Nor:155±19; Hyp:167±13 (beats·min); p<0.05), no other significant between-group differences were noted during the cycling and skiing protocols. In summary, these data suggest similar cardiorespiratory responses to submaximal hypoxic cycling and simulated skiing in adults and children.