The lysis of occluding arterial thrombi in dogs with intravenous injections of CA-7 (fibrinolytic enzyme from Aspergillus oryzae) is described. The treatments were conducted over a 3-day period and resulted in lysis of 75% of the arterial thrombi. This is compared with the results of an earlier study in which dogs received massive single doses of the enzyme. The therapeutic effectiveness is similar in both series. The 3-day treatment has advantages in reduced toxicity and increased safety.
Procedures are described for the purification of a thrombolytic fungal protease. These include precipitation with either lignin or tannin, removal of lignin or tannin with acetone, treatment with Benzathine, dialysis, passage through DEAE-Sephadex A-50 and Sephadex G-50, and finally lyophilization. The final product has a molecular weight of about 22,000 and is substantially pure. Amino acid analysis reveals a total absence of cysteine and cystine. The anomalous retention by Sephadex G-50 is discussed.
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