SUMMARY We studied larval development and acquisition of metabolic resources in Drosophila melanogaster selected for adult desiccation resistance. Desiccation-selected (D) flies had longer developmental times in comparison with control populations, resulting in significantly higher body mass. No differences were found in larval growth rates, suggesting that increased body mass results from the extended larval feeding period. Larvae from both D and control lines molted from second to third instar 81-84 h after egg laying,whereas D larvae pupated 5-6 h later than controls. This indicates that selecting adults for desiccation resistance results in longer third larval instar. Newly eclosed D flies had higher carbohydrate and water contents in comparison with control populations. No differences were found in body mass,water or metabolite contents of newly eclosed selected and control flies after larvae were prevented from further feeding from 96 h after egg laying onwards. This shows that differences in accumulated carbohydrate and water stores are a result of evolved differences in the duration of third larval instar. The contribution of third instar feeding to desiccation resistance of adult selected flies is higher than that of controls. Thus, selection for adult stress resistance has resulted in correlated changes in larval physiology.

We used experimental evolution to test the ‘melanism-desiccation’ hypothesis, which proposes that dark cuticle in several Drosophila species is an adaptation for increased desiccation tolerance. We selected for dark and light body pigmentation in replicated populations of D. melanogaster and assayed several traits related to water balance. We also scored pigmentation and desiccation tolerance in populations selected for desiccation survival. Populations in both selection regimes showed large differences in the traits directly under selection. However, after over 40 generations of pigmentation selection, dark-selected populations were not more desiccation-tolerant than light-selected and control populations, nor did we find significant changes in mass or carbohydrate amounts that could affect desiccation resistance. Body pigmentation of desiccation-selected populations did not differ from control populations after over 140 generations of selection, although selected populations lost water less rapidly. Our results do not support an important role for melanization in Drosophila water balance.

No abstract

We used experimental evolution to test the ‘melanism-desiccation’ hypothesis, which proposes that dark cuticle in several Drosophila species is an adaptation for increased desiccation tolerance. We selected for dark and light body pigmentation in replicated populations of D. melanogaster and assayed traits related to water balance. We also scored pigmentation and desiccation tolerance in populations selected for desiccation survival. Populations in both selection regimes showed large differences in the traits directly under selection. However, after over 40 generations of pigmentation selection, dark-selected populations were not more desiccation-tolerant than light-selected and control populations, nor did we find significant changes in carbohydrate amounts that could affect desiccation resistance. Body pigmentation of desiccation-selected populations did not differ from control populations after over 140 generations of selection. Our results do not support an important role for melanization in Drosophila water balance.

No abstract