Objective: To estimate the prevalence of SARS-CoV-2 antibodies in an asymptomatic population in the state of Sergipe, Brazil. Methods: This cross-sectional study with stratified sampling (sex and age) includeD serological immunofluorescent tests for IgM and IgG on samples from 3 046 asymptomatic individuals. Sample collection was performed in wet-markets of the 10 most populous cities of Sergipe, Brazil. Exclusion criteria included symptomatic individuals and health workers. The presence of comorbidities was registered. Results: Of the 3 046 participants, 1 577 (51.8%) were female and 1 469 (48.2%) were male; the mean age was 39.76 (SD 16.83) years old. 2 921 tests were considered valid for IgM and 2 635 for IgG. Of the valid samples, 347 (11.9% [CI 10.7%–13.1%]) tested positive for IgM and 218 (8.3% CI 7.2%–9.4%]) tested positive for IgG. Women over 40 had the highest prevalence for IgM (group C, p=0.006; group D p=0.04). The capital Aracaju displayed the highest prevalence for both antibodies; 83 (26.3% [CI 21.6%-31.6%]) tested positive for IgM and 35 (14.6% [CI 10.4%-19.7%]) for IgG. The most prevalent comorbidities were hypertension (64/123 individuals) and diabetes (29/123). Conclusions: A high prevalence of SARS-CoV-2 antibodies was found among asymptomatic persons in Sergipe. Women over 40 showed the highest rates. The capital, Aracaju, displayed the highest seroprevalence. Surveys like this one are important to understand how the virus spreads and to help authorities to plan measures to control it. Repeated serologic testing are required to track the progress of the epidemic.
ABSTRACT. Current experiment identified the height of signal grass {Urochloa decumbens (Stapf) R. D. Webster cv. Basilisk [syn. Brachiaria decumbens Stapf cv. Basilisk]} at the beginning of deferment that provided an appropriate pasture structure and potential selectivity by cattle on deferred pastures. Four pasture heights at the beginning of deferment (10, 20, 30 and 40 cm) and two forage samples (available on pasture and simulated grazing) were studied. The experimental design was set in completely randomized blocks, with two replications, in a split-plot arrangement. Higher percentage of live leaf blades and lower percentage of live stems and senescent forage were recorded in the forage sample from simulated grazing. The increase in pasture height increased the percentage of senescent forage and reduced the percentage of live leaf blades in forage samples. Pasture height at the beginning of deferment did not affect the potential selectivity index by cattle for the percentage of live leaf blade. The potential selectivity index varied quadratically for the percentage of live stems and increased linearly for the percentage of senescent forage with pasture height. A 10-to-20 cm reduction in pasture height at the beginning of deferment improved the structure of deferred signal grass and optimized selectivity by cattle.Keywords: Brachiaria decumbens, stem, leaf, senescent forage, grazing.Altura do pasto no início do diferimento como determinante da estrutura do capimbraquiária e da seletividade potencial de bovinos RESUMO. Objetivou-se identificar a altura do capim-braquiarinha {Urochloa decumbens (Stapf) R. D. Webster cv. Basilisk [syn. Brachiaria decumbens Stapf cv. Basilisk]} no início do diferimento que possibilite adequada estrutura de pasto e seletividade potencial dos bovinos em pastagens diferidas. Foram estudadas quatro alturas do pasto no início do diferimento (10, 20, 30 e 40 cm) e duas amostras de forragem (disponível na pastagem e de pastejo simulado). O delineamento experimental foi em blocos completos casualizado com duas repetições, em esquema de parcela subdividida. Foi registrada maior porcentagem de lâmina foliar viva e menor de colmo vivo e forragem senescente na amostra de forragem em pastejo simulado. O incremento na altura do pasto aumentou o porcentual de forragem senescente e reduziu a porcentagem de lâmina foliar viva nas amostras de forragem. A altura do pasto no início do diferimento não influenciou o índice de seletividade potencial de bovinos para a porcentagem de lâmina foliar viva. O índice de seletividade potencial variou quadraticamente para a porcentagem de colmo vivo e aumentou linearmente para a porcentagem de forragem senescente com a altura do pasto. A redução da altura do pasto para 10 a 20 cm no início do diferimento melhora a estrutura do capim-braquiária diferido e otimiza a seletividade dos bovinos.Palavras-chave: Brachiaria decumbens, colmo, folha, forragem senescente, pastejo.
Purpose Several interactions exist between the GH/IGF axis and the immune system, including effects on innate immunity and humoral and cellular response. Acquired GH deficiency (GHD) has been recently proposed as a risk factor for severity of COVID-19 infections. However, acquired GHD is often associated to other factors, including pituitary tumors, surgery, radiotherapy, and additional pituitary hormones deficits and their replacements, which, together, may hinder an accurate analysis of the relationship between GHD and COVID-19. Therefore, we decided to assess the seroprevalence of SARS-CoV-2 antibodies and the frequency of symptomatic cases of COVID-19 in adults subjects with untreated isolated GHD (IGHD) due to a homozygous null mutation in the GHRH receptor gene. Methods A cross-sectional study was carried out in 27 adult IGHD subjects and 27 age- and gender-matched local controls. Interview, physical examination, bio-impedance, hematological and SARS-CoV-2 IgM and IgG antibodies were analyzed. Results There was no difference in the prevalence of positivity of anti-SARS-CoV-2 IgM and IgG antibodies between the two groups. Conversely, no IGHD individual had a previous clinical diagnosis of COVID-19 infection, while 6 control subjects did ( p = 0.023). Conclusion The production of anti-SARS-CoV-2 antibodies was similar between IGHD subjects due to a GHRH receptor gene mutation and controls, but the evolution to symptomatic stages of the infection and the frequency of confirmed cases was lower in IGHD subjects than in GH sufficient individuals.
The effects of supplement doses (0, 1, 2 and 3 kg ha-1 day-1) and grazing periods (1, 28, 59, 89 and 103 days) on the B. decumbens cv. Basilisk structure and cattle production in deferred pastures were evaluated. The design was a randomised complete block design with two replications. The sward height and the total forage and stem masses decreased with grazing period. The crude protein (8.12%) and in vitro organic matter digestibility (52.74%) were not affected by grazing period. The fibre content in neutral and acid detergent increased linearly with the grazing period. The average daily gain of cattle ranged from 0.419 to 1.019 kg animal-1 day-1; the stocking rate ranged from 2.7 to 3.9 animal unit ha-1; and the average gain per area ranged from 2.7 to 7.9 kg ha-1 day-1, with the maximum values obtained with the highest dose of concentrate. Concentrate supplementation increases cattle production in deferred pastures. During the use of deferred pasture in winter there are decreases in the quantity and quality of forage, as well as limitations in its structure.
Background: The exponential growth of COVID-19 cases in Brazil is overloading health systems with overcrowding of hospitals and overflowing intensive care units. Increasing infection rates in health professionals can lead to the collapse of the health system and further worsen the pandemic. The aim of this study was to evaluate the seroprevalence of IgM and IgG for SARS-CoV-2 in health workers in Sergipe, Brazil. Methods: The targeted tests involved health professionals working on the front line to combat COVID-19. The samples were collected in the month of June, in six hospital units in the state of Sergipe. Results: 471 health professionals were tested. Of these, 28 workers (5.95%) tested positive for IgM and 64 (13.59%) tested positive for IgG. 9 workers (1.91%) tested positive for IgM and were also positive for IgG. Discussion: Health workers must be monitored constantly, because if they are infected, they can spread the virus to colleagues, hospitalized patients and even family members. Conclusion: Knowing the prevalence of antibodies to the virus in health workers is an important measure of viral spread control. Keywords: SARS-CoV-2, COVID-19, Health workers, Immunofluorescence assays.
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