Carbon metabolism in higher plants is a basic physiological metabolism, and carbon allocation and conversion require the activity of various enzymes in metabolic processes that alter the content and overall composition of sugars in the sink organ. However, it is not known how various enzymes affect carbon metabolism when tomato plants are subjected to water stress or treated with potassium. Although the process of carbon metabolism is very complex, we used the carbon conversion rate to compare and analyze the enzyme activities related to sugar metabolism and find out which carbon conversion rate are the most important. Results showed that water stress and potassium increased carbon import flux in the fruit, which was beneficial to carbon accumulation. Water deficit increased the activity of sucrose synthase (SuSy) and starch phosphorylase (SP) and decreased the activity of sucrose phosphate synthase (SPS) and adenosine diphosphate glucose pyrophosphorylase (AGPase) in the source. Water stress increased the activity of acid invertase (AI), SuSy and SP but decreased the activity of AGPase in the sink. Potassium modified the balance of enzymes active in sugar and starch metabolism by increasing the activity of AI, SuSy, SPS and SP and significantly decreasing the activity of AGPase, resulting in increase of hexose. Canonical correlational analysis revealed that the carbon conversion rate was mainly affected by the relative rate of conversion of sucrose to fructose and glucose [p1(t)] and glucose to starch [p5m(t)]. SuSy and AGPase had the greatest effect on enzyme activity in the fruit; respectively regulated p1(t) and p5m(t).
Carbohydrate concentrations in fruit are closely related to the availability of water and mineral nutrients. Water stress and minerals alter the assimilation, operation, and distribution of carbohydrates, thereby affecting the fruit quality. The SUGAR model was used to investigate the carbon balance in tomato fruit during different growth stages when available water was varied and potassium added. Further, we quantitatively studied the distribution of photoassimilates such as structural carbohydrates, soluble sugars, and starch in fruit and evaluated their response to water and potassium supply. The results revealed that the carbon allocation and transformation dynamically changed during the all growth stages; in fact, variation in carbon content showed similar trends for different water along with potassium treatments, carbon allocation during the early development stages was mainly to starch and structural carbon compounds. The relative rate of carbon conversion of soluble sugars to structural carbon compounds (k 3) and of soluble sugars to starch (k 5m) peaked during the initial stage and then dropped during fruit growth and development stages. Carbon was primarily allocated as soluble sugars and starch was converted to soluble sugars at fruit maturation. k 3 (t) and k 5m (t) approached zero at the end of the growth stage, mainly due to sugar accumulation. Potassium application can significantly raise carbon flows imported (C supply) from the phloem into the fruit and thus increased carbon allocation to soluble sugars over the entire growth period. Potassium addition during the fruit maturation stage decreased the content of starch and other carbon compounds. Water deficit regulated carbon allocation and increased soluble sugar content but reduced structural carbon content, thereby improving fruit quality.
Background The increasing concentration of atmospheric CO2 not only affects the growing environment of crops but also aggravates the global greenhouse effect and further aggravates the problem of water shortage. The combined effect of water deficit and potassium (K) application has not been widely studied. Aims A pot experiment was conducted to investigate the effect of deficit irrigation (DI) and K application at different growth stages on carbon allocation and enzyme activities related to sugar metabolism. Methods Tomatoes were transplanted and planted on April 26, 2017 and harvested on August 15, 2017. Four irrigation regimes were implemented with two water levels (full irrigation‐W and DI‐W/2) in different growth stages, and each water treatment was equally divided into two subgroups: with K (K1) and without K (K0). Fruits from the first to fourth trusses of the tomato plants were sampled. Tomato growth, carbon allocation, and related enzyme activities were measured. Results The fresh weight (FW), dry weight, and relative growth rate of dry mass were sensitive to irrigation amount under K fertilization, enhancing the promotion effect of irrigation on fruit. Meanwhile, carbon allocation was sensitive to irrigation amount under K regime. Sucrose synthase (SuSy), acid invertase (AI), and sucrose phosphate synthase (SPS) were also highly sensitive to irrigation amount under K application condition. Starch phosphorylase displayed a quadratic parabola for irrigation amount, and adenosine diphosphate glucose pyrophosphorylase (AGPase) was highly sensitive to irrigation amount without K fertilization. Carbon in the form of other carbohydrates, carbon in the form of soluble sugar (Csol), and fruit water content were the factors that had the greatest influence on the principal components. Classification by K‐means algorithm and canonical correlation analysis showed that FW, fructose, sucrose, and starch could be used as significant indicators of the dry matter components of the fruit for the treatment without K. In the case of K regime, SuSy, AGPase, AI, and Csol could be used as a significant indicator of the correlation analysis of carbon metabolism activity. Conclusions The factors related to the improvement of fruit quality and carbon allocation by deficient irrigation and K application were explored. Water stress changed the distribution of photosynthetic carbon between starch and soluble sugar. K application further changed the balance between soluble sugars and other compounds. In particular, it significantly increased the carbon content of soluble sugars and decreased that of other compounds. AI and SuSy are key enzymes affecting carbon metabolism under water‐deficient conditions.
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