Peaches mainly produced in China are a source of food-derived proteins, but much of it is wasted. To make full use of the peaches and peaches related resources, we extracted proteins, including albumin, globulin, gliadin, and glutelin, from defatted Tibet wild peach kernels using Osborne method to study their angiotensin I-converting enzyme (ACE) inhibiting activity, which is an active ingredient for hypertension treatment. The different enzymatic products of these extracts were further separated by ultrafiltration membranes and reversed phase high-performance liquid chromatography (RP-HPLC). The component K2 originated from globulin hydrolysate by alcalase treatment showed the highest ACE inhibiting activity (IC 50 0.78 mg/mL). Through further analyzing the Mass spectrometry results of K2 and searching the BIOEP database, the specific dipeptide AH (consisted of Ala and His) has been identified as the ACE inhibitory peptide, providing a potential for using Tibet peach kernelderived peptides as an ingredient in functional food to control hypertension.
Summary In this work, the release pattern of bound phenolics in salt‐dried bamboo shoots (SDBS), steamed dried bamboo shoots (TDBS), fumigation dried bamboo shoots (UDBS) and fermentation dried bamboo shoots (FDBS) during simulated in vitro digestion was studied. The result exhibited that polyphenols in dried bamboo shoots (DBS) mainly existed in the form of bound phenolics, amongst them the content of FDBS was the highest, followed by SDBS and TDBS, and UDBS was the lowest. In addition, release amounts of bound phenolics in SDBS, TDBS, UDBS and FDBS after gastrointestinal digestion were significantly higher than that after gastric digestion, accounting for 19.61%, 19.40%, 45.64% and 19.66% of the total, respectively. Moreover, IC50 values for scavenging ability of DPPH and ABTS radicals by gastric and gastrointestinal digestion supernatant for DBS were in a descending order: FDBS, UDBS, SDBS and TDBS. Rutin, p‐coumaric and ferulic acid were discovered to be the predominant compounds in digestion supernatant. Trustworthy, fluorescence microscope pictures of DBS at different digestion stages further confirmed the release regularity of bound phenolics. Therefore, these findings could provide a theoretical support for the optimal processing of DBS.
Pseudomonas aeruginosa is a conditional Gram-negative pathogen that produces extracellular virulence factors that can lead to bloodstream invasion, severely harm tissues, and disseminate bacteria, ultimately leading to various diseases. In this study, lactic acid bacteria (LAB) with strong antagonistic ability against P. aeruginosa were screened, and the regulatory mechanism of LAB against P. aeruginosa was evaluated. The results showed that the three selected LAB strains had strong inhibition ability on the growth, biofilm formation, and pyocyanin expression of P. aeruginosa and a promoting effect on the expression of autoinducer-2. Among them, Lactipantibacillus plantarum (Lp. plantarum) LPyang is capable of affecting the metabolic processes of P. aeruginosa by influencing metabolic substances, such as LysoPC, oxidized glutathione, betaine, etc. These results indicate that LPyang reduces the infectivity of P. aeruginosa through inhibition of its growth, biofilm formation, pyocyanin expression, and regulation of its metabolome. This study provides new insights into the antagonistic activity of Lp. plantarum LPyang against P. aeruginosa.
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