The diagnostic value and suitability of circulating miRNAs for the detection of hepatocellular carcinoma have been inconsistent in the literature. A meta-analysis is used to systematically evaluate the diagnostic value of circulating miRNAs. Eligible studies were selected and the heterogeneity was assessed by subgroup analysis, meta-regression, and publication bias. After strictly and comprehensive screening, the source methods, internal reference and the cut-off values of the included miRNAs were first listed. Circulating miRNAs demonstrated a relatively good diagnostic value in hepatocellular carcinoma, In the subgroup analysis, diagnosis odds ratio showed a higher accuracy with multiple miRNAs than with a single miRNA as well as with serum types than plasma types. In addition, although miRNAs have many expression patterns, the high frequency expression miRNAs (miR-21, miR-199 and miR-122) might be more specific for the diagnosis of hepatocellular carcinoma.The sources of heterogeneity might be related to the number of miRNAs and the specimen types in meta-regression. Furthermore, it’s surprised that the pooled studies were first demonstrated publication bias (P < 0.05). In conclusion, multiple miRNAs in serum have a better diagnostic value, and the publication bias was stable. To validate the potential applicability of miRNAs in the diagnosis of hepatocellular carcinoma, more rigorous studies are needed to confirm these conclusions.
Resveratrol (Res) is a natural active antioxidant that is effective in relieving inflammatory bowel disease (IBD). However, the specific mechanism for its function is unknown. In our study, dextran sodium sulfate (DSS)-induced mouse IBD disease model was constructed. All mice were randomly divided into three groups. The treatment effects of resveratrol on IBD were evaluated by observing the body weight, fecal traits, colon/ spleen gross appearance, tissue hematoxylin-eosin (H&E)/immunohistochemistry (IHC) and inflammatory factors. The expression of small ubiquitin-like modifier protein 1 (SUMO1) and its Wnt/β-catenin pathwayrelated genes was analyzed by IHC, Western blot, Real-time PCR (RT-PCR) and Immunofluorescence. The outcome indicated that resveratrol treatment significantly relieved the symptoms of IBD. The expression level of anti-inflammatory cytokines was increased while that of pro-inflammatory cytokines was decreased in both colon and spleen tissues of resveratrol-treated mice. SUMO1 expression and Wnt/β-catenin pathway were suppressed in colon and spleen tissues of IBD mice treated with resveratrol. In addition, we provided evidence that resveratrol inhibited SUMO1 and β-catenin expression and their nuclear localization in human colonic epithelial cell line (FHC). Moreover, we found that SUMO1 and β-catenin had higher expression levels in colorectal cancer patients than in health and colitis patients. In conclusions, resveratrol alleviates DSSinduced IBD by modulating SUMO1 through Wnt/β-catenin pathway.
Human umbilical cord mesenchymal
stem cell-derived exosome (hucMSC-Ex)
plays an important role in tissue repair and immunomodulation, leading
to the mitigation of inflammatory bowel disease. However, the preventive
function of hucMSC-Ex in the onset and progression of colitis-associated
colon cancer (CAC) is poorly understood. In the current study, dextran
sodium sulfate/azoxymethane-induced colitis mouse model was established,
and the mice disease activity index, body weight, colon length, tumor
counts, survival curve, tissue H&E/immunohistochemistry, and cytokines
expression were analyzed to evaluate the effects of hucMSC-Ex on CAC.
In addition, miR-146a mimics were transfected into colonic epithelial
cells (fetal human cells) to evaluate their role in the hucMSC-Ex-mediated
regulation of SUMO1. The results showed that hucMSC-Ex inhibits the
expression of SUMO1 to reduce the process of CAC progression. Further
analysis indicated that miR-146a targets and inhibits SUMO1 expression
and its binding to β-catenin. In conclusion, our findings showed
that hucMSC-Ex is effective in alleviating the deterioration of colitis
via the miR-146a-mediated inhibition of SUMO1, which is crucial in
this disease process.
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