Releasing sterile or incompatible male insects is a proven method of population management in agricultural systems with the potential to revolutionize mosquito control. Through a collaborative venture with the “Debug” Verily Life Sciences team, we assessed the incompatible insect technique (IIT) with the mosquito vector Aedes aegypti in northern Australia in a replicated treatment control field trial. Backcrossing a US strain of Ae. aegypti carrying Wolbachia wAlbB from Aedes albopictus with a local strain, we generated a wAlbB2-F4 strain incompatible with both the wild-type (no Wolbachia) and wMel-Wolbachia Ae. aegypti now extant in North Queensland. The wAlbB2-F4 strain was manually mass reared with males separated from females using Verily sex-sorting technologies to obtain no detectable female contamination in the field. With community consent, we delivered a total of three million IIT males into three isolated landscapes of over 200 houses each, releasing ∼50 males per house three times a week over 20 wk. Detecting initial overflooding ratios of between 5:1 and 10:1, strong population declines well beyond 80% were detected across all treatment landscapes when compared to controls. Monitoring through the following season to observe the ongoing effect saw one treatment landscape devoid of adult Ae. aegypti early in the season. A second landscape showed reduced adults, and the third recovered fully. These encouraging results in suppressing both wild-type and wMel-Ae. aegypti confirms the utility of bidirectional incompatibility in the field setting, show the IIT to be robust, and indicate that the removal of this arbovirus vector from human-occupied landscapes may be achievable.

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The newly identified "Forests of East Australia" Global High Biodiversity Hotspot corresponds with two World Wildlife Fund (WWF) Ecoregions: the Eastern Australian Temperate Forests and Queensland's Tropical Rain forests. The region contains more than 1,500 endemic vascular plants, meeting the criterion for global biodiversity significance, and more than 70% of natural areas have been cleared or degraded, meeting the criterion for a hotspot. The hotspot, although covering a large latitudinal range (15.5 -35.6 South), has a predominantly summer rainfall pattern with increasing rainfall seasonality northwards into tropical areas of north Queensland. It covers large tracts of elevated tablelands and drier inland slopes, particularly in New South Wales, where it extends inland beyond the New England Tablelands and the Great Dividing Range. Varied soils result in a mosaic pattern of vegetation. Sclerophyllous communities dominated by Australia's iconic plant, the gum-tree (Eucalyptus species), are the most prevalent vegetation type. Significant areas of rain forest exist throughout the region, much of which has persisted continuously since Gondwanan times, providing a rich living record of evolution over more than 100 million years. The human population of the hotspot as of 2006 was over nine million, with a population density of 36 people per square kilometer, mainly concentrated along the coast. About 18% of the land area is under some form of formal protection for its natural values. Gaps in the protected area network include some centers of plant endemism and some areas of critical habitat for threatened species. Whole of landscape conservation initiatives are enhancing connectivity throughout the Great Dividing Range through voluntary protection and restoration programs.

BackgroundWidespread uptake of DNA barcoding technology for vascular plants has been slow due to the relatively poor resolution of species discrimination (∼70%) and low sequencing and amplification success of one of the two official barcoding loci, matK. Studies to date have mostly focused on finding a solution to these intrinsic limitations of the markers, rather than posing questions that can maximize the utility of DNA barcodes for plants with the current technology.Methodology/Principal FindingsHere we test the ability of plant DNA barcodes using the two official barcoding loci, rbcLa and matK, plus an alternative barcoding locus, trnH-psbA, to estimate the species diversity of trees in a tropical rainforest plot. Species discrimination accuracy was similar to findings from previous studies but species richness estimation accuracy proved higher, up to 89%. All combinations which included the trnH-psbA locus performed better at both species discrimination and richness estimation than matK, which showed little enhanced species discriminatory power when concatenated with rbcLa. The utility of the trnH-psbA locus is limited however, by the occurrence of intraspecific variation observed in some angiosperm families to occur as an inversion that obscures the monophyly of species.Conclusions/SignificanceWe demonstrate for the first time, using a case study, the potential of plant DNA barcodes for the rapid estimation of species richness in taxonomically poorly known areas or cryptic populations revealing a powerful new tool for rapid biodiversity assessment. The combination of the rbcLa and trnH-psbA loci performed better for this purpose than any two-locus combination that included matK. We show that although DNA barcodes fail to discriminate all species of plants, new perspectives and methods on biodiversity value and quantification may overshadow some of these shortcomings by applying barcode data in new ways.

This study was to determine the shelf life of minimally processed honeydew melon, kiwifruit, papaya, pineapple, and cantaloupe stored at 4°C. Sensory assessments were carried out at 3-day intervals by highly trained panels-until the end of shelf life. Microbiblogical courts were made immediatelv after dicing fruit and at the end of shelf life. Results indicated that 60th the lengih of shelf life and type of spoilage were related to fruit species. Minimally processed fruit had longer shelf life at 4°C than at temperatures recommended for whole fruit when these were greater than 4°C. Spoilage of 4'C-stored kiwifruit, papaya and pineapple pieces was not a consequence of microbial growth.

Pressures of the order of 100 MNm'* applied for 2.5 min or longer to postrigor muscle heated to 40-60"~ improved the tenderness of the meat when subsequently cooked. The magnitude of the effect depended on the intensity and duration of pressurization, and the temperature attained by the meat during pressurization. As judged by taste panel assessment and by shear values of the cooked meat, the properties of pressure-heat treated postrigor muscle approximated those of prerigor pressurized muscle. The process is effective in overcoming toughness associated with contracted muscle. It is suggested that the treatment operates on the myofibrillar component of toughness.

In tropical forests, natural disturbance creates opportunities for species to claim previously utilized space and resources and is considered an important mechanism in the maintenance of species diversity. However, ecologists have long recognized that disturbance also promotes exotic plant invasions. Cyclones cause extensive defoliation, loss of major branches and multiple tree falls, resulting in a significantly more open canopy and increased light and heat levels in the understorey. The widespread and massive disturbance caused by cyclones provides ideal conditions for rapid recruitment and spread of invasive species. The ecological roles of invasive species in rainforest habitats following such a severe disturbance are poorly understood. Severe category 4 Cyclone Larry crossed the North Queensland coast in March 2006 causing massive disturbance to rainforest habitats from Tully to Cairns and west to the Atherton Tablelands. We established 10 plots in an area extensively damaged by this cyclone near El Arish in North Queensland. On each plot nine 2 × 2 m quadrats were established with three quadrats per plot in each of the following treatments: (i) complete debris removal down to the soil layer, (ii) removal of coarse woody debris only, and (iii) uncleared. We monitored recruitment, growth and mortality of all native and invasive species in the 90 quadrats every 3 months since the cyclone. Here we present the recruitment dynamics of invasive species across the study area in relation to the level of disturbance, the type of quadrat treatment, and the diversity and abundance of the native recruiting flora over the first 12 months post‐cyclone. Our results suggest that invasive species will mostly comprise a transient component of the flora in the early stages of the successional response. However, some species may have longer‐term effects on the successional trajectory of the rainforest and future forest composition and structure.

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