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Please cite this article as: Alfaro, Andrea C., Young, Tim, Ganesan, Annapoorna Maitrayee, Regulatory effects of mussel (Aulacomya maoriana Iredale 1915) larval settlement by neuroactive compounds, amino acids and bacterial biofilms, Aquaculture (2011), doi: 10.1016/j.aquaculture.2011.08.038 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Pseudoalteromonas sp. AMGP1 were tested for their abilities to induce larval settlement. Toxicity effects of each treatment also were simultaneously identified by recording larval mortalities. Results indicate that all chemicals used induced larvae to settle, with acetylcholine being the most effective (~24% at 10 -6 M compared to <2% in control assays). Toxicities of treatment compounds were low at optimal settlement inducing concentrations, except for L-Tryptophan (~32%) and GABA (~59%). Our data suggest that catecholamines (and their precursors) play an important role in the biochemical mechanisms of settlement for A. maoriana. While serotonin precursors did induce low levels of larval settlement at some concentrations, high toxicity responses to 5-HTP at 10 -5 M, combined with complete settlement inhibition indicate A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPTthat the mechanism of action may be more complex than can be elucidated in this study. Larval settlement responses to bacterial treatments were low for planktonic and biofilm phases across all three strains, and settlement inhibition was observed when larvae were exposed to biofilm exudates of all bacterial strains. Comparisons of A. maoriana responses to other endemic and worldwide distributed mussel species are provided as a means to highlight potential evolutionary differences in chemoreception mechanisms. Highlights 1.Mussel larval settlement responses were tested with neuroactive compounds amino acids, and bacterial biofilms.2. All chemicals induced larvae to settle, with acetylcholine being the most effective.3. Catecholamines (and their precursors) play an important role in the biochemical mechanisms of settlement for A. maoriana. Settlement responses to serotonin precursors require further investigationsince there were high toxicity responses.5. Larval settlement responses to bacterial treatments were low for planktonic and biofilm phases, and exposure to biofilm exudates resulted in settlement inhibition.

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