Globally, the Blood Donor Unit service has had a significant impact during the Coronavirus Disease-19 (COVID-19) pandemic. A significant impact occurred on donor recruitment due to the many considerations for donors during the pandemic. Changes in donor characteristics data before the pandemic and during the pandemic may occur. Therefore, researchers analyzed the impact of the pandemic on donors at Blood Donor Unit (Indonesian Red Cross, Semarang City, 2019 to 2021. Subjects and Methods: A study with a cross sectional design was conducted at Blood Donor Unit PMI Semarang. Secondary data collection consisting of voluntary blood donor form data from 2019 to 2021. The data were analyzed by One-Way Anova and Kruskal-Wallis with SPSS version 25. The study showed a significant decrease in age categories, namely < 18 years, 18-24 years and 25 -44 years and repeat donors in 2021 with 2019 and 2020 with 2019 (p < 0.05). There was a significant decrease in first-time donors in 2021 with 2019 and in 2020 with 2019 (p < 0.001). However, there was an increase in donors in the >60 year age category in 2020 and 2021 by 18.6% and 42% compared to 2019 although not statistically significant.
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