Key points• A new class of interstitial cells, PDGFRα + cells, is distributed densely in the proximal stomachs of mice.• PDGFRα + cells express the molecular apparatus necessary for transduction of inputs from enteric inhibitory motor neurons.• • Spontaneous Ca 2+ transients may be a means of regulating basal excitability of fundus muscles and release of purines from motor neurons may contribute to the control of pressure during filling in the proximal stomach.Abstract Platelet-derived growth factor receptor α positive (PDGFRα + ) cells are suggested to mediate purinergic inputs in GI muscles, but the responsiveness of these cells to purines in situ has not been evaluated. We developed techniques to label and visualize PDGFRα + cells in murine gastric fundus, load cells with Ca 2+ indicators, and follow their activity via digital imaging. Immunolabelling demonstrated a high density of PDGFRα + cells in the fundus. Cells were isolated and purified by fluorescence-activated cell sorting (FACS) using endogenous expression of enhanced green fluorescent protein (eGFP) driven off the Pdgfra promoter. Quantitative PCR showed high levels of expression of purinergic P2Y1 receptors and SK3 K + channels in PDGFRα + cells. Ca 2+ imaging was used to characterize spontaneous Ca 2+ transients and responses to purines in PDGFRα + cells in situ. ATP, ADP, UTP and β-NAD elicited robust Ca 2+ transients in PDGFRα + cells. Ca 2+ transients were also elicited by the P2Y1-specific agonist (N )-methanocarba-2MeSADP (MRS-2365), and inhibited by MRS-2500, a P2Y1-specific antagonist. Responses to ADP, MRS-2365 and β-NAD were absent in PDGFRα + cells from P2ry1 (−/−) mice, but responses to ATP were retained. Purine-evoked Ca 2+ transients were mediated through Ca 2+ release mechanisms. Inhibitors of phospholipase C (U-73122), IP3 (2-APB), ryanodine receptors (Ryanodine) and SERCA pump (cyclopiazonic acid and thapsigargin) abolished Ca 2+ transients elicited by purines. This study provides a link between purine binding to P2Y1 receptors and activation of SK3 channels in PDGFRα + cells. Activation of Ca 2+ release is likely to be the signalling mechanism in PDGFRα + cells responsible for the transduction of purinergic enteric inhibitory input in gastric fundus muscles.
Background/AimsInterstitial cells of Cajal (ICC) play important functions in motor activity of the gastrointestinal tract. The role of ICC as pacemakers is well established, however their participation in neurotransmission is controversial. Studies using mutant animals that lack ICC have yielded variable conclusions on their importance in enteric motor responses. The purpose of this study was to:(1) clarify the role of intramuscular ICC (ICC-IM) in gastric motor-neurotransmission and (2) ConclusionsThis study demonstrates that ICC-IM mediate nitrergic inhibitory neurotransmission in the fundus and provides evidence of plasticity changes in neuronal responses that may explain discrepancies in previous functional studies which utilized mutant animals to examine the role of ICC-IM in gastric enteric motor responses.
Background/AimsInterstitial cells of Cajal (ICC) play important functions in motor activity of the gastrointestinal tract. The role of ICC as pacemakers is well established, however their participation in neurotransmission is controversial. Studies using mutant animals that lack ICC have yielded variable conclusions on their importance in enteric motor responses. The purpose of this study was to:(1) clarify the role of intramuscular ICC (ICC-IM) in gastric motor-neurotransmission and (2) ConclusionsThis study demonstrates that ICC-IM mediate nitrergic inhibitory neurotransmission in the fundus and provides evidence of plasticity changes in neuronal responses that may explain discrepancies in previous functional studies which utilized mutant animals to examine the role of ICC-IM in gastric enteric motor responses.
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