The development of carrier-antitumor complexes is aimed at reducing toxicity to normal cells and increasing antitumor activity. The aim of this paper was to study the antioxidant activity and cytotoxicity of the complex of poly-γ-glutamic acid with Doxorubicin in different ratios on different cultures of tumor cells in comparison with the toxicity of Doxorubicin alone.Poly-γ-glutamic acid (PGA) is anionic biopolymer produced by Bacillus licheniformis M 20G. Cell lines were A549, LL, P3HR1, MDBK. Antioxidant activity was determined with DPPHtest and cytotoxicity with MTT-test.PGA-Dox complexes showed antioxidant activity up to 25%. The complex showed at least two times higher toxicity compared with Doxorubicin against A549 cells after 48 h: the CC 50 was 1.5 µg/ml for Doxorubicin and 0.31 -0.72 µg/ml for PGA-Dox. In P3HR1 cells the CC 50 for Doxorubicin was 0.68 µg/ml, and in the PGA-Dox complex 0.31 -0.68 µg/ml. In Lewis carcinoma cells (LL), the complexes were more effective after 24 h (the CC 50 were 0.92 -1.06 µg/ml for PGA-Dox, 1.14 µg/ml for Dox). For non-tumor cells of MDBK Doxorubicin was as toxic as for tumor cells (CC 50 was 1.24 µg/ml), and the toxicity of the PGA-Dox complexes were significantly lower (3.38-68.40 µg/ml).The results presented in this work demonstrated that the PGA-Dox complexes exhibited greater toxicity to tumor cells than Doxorubicin and were less toxic to cells of non-tumor origin.
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