The physics of star formation and the deposition of mass, momentum, and energy into the interstellar medium by massive stars (‘feedback’) are the main uncertainties in modern cosmological simulations of galaxy formation and evolution1, 2. These processes determine the properties of galaxies3, 4, but are poorly understood on the ≲100 pc scale of individual giant molecular clouds (GMCs)5, 6 resolved in modern galaxy formation simulations7, 8. The key question is why the timescale for depleting molecular gas through star formation in galaxies (tdep ≈ 2 Gyr)9, 10 exceeds the dynamical timescale of GMCs by two orders of magnitude11. Either most of a GMC’s mass is converted into stars over many dynamical times12, or only a small fraction turns into stars before the GMC is dispersed on a dynamical timescale13, 14. Here we report our observation that molecular gas and star formation are spatially decorrelated on GMC scales in the nearby flocculent spiral galaxy NGC300, contrary to their tight correlation on galactic scales5. We demonstrate that this de-correlation implies rapid evolutionary cycling between GMCs, star formation, and feedback. We apply a novel statistical method15, 16 to quantify the evolutionary timeline and find that star formation is regulated by efficient stellar feedback, driving GMC dispersal on short timescales (~1.5 Myr) due to radiation and stellar winds, prior to supernova explosions. This feedback limits GMC lifetimes to about one dynamical timescale (~10 Myr), with integrated star formation efficiencies of only 2–3%. Our findings reveal that galaxies consist of building blocks undergoing vigorous, feedback-driven lifecycles, that vary with the galactic environment and collectively define how galaxies form stars. Systematic applications of this multi-scale analysis to large galaxy samples will provide key input for a predictive, bottom-up theory of galaxy formation and evolution.

We present MUSE integral field data of two HII region complexes in the Large Magellanic Cloud (LMC), N and N . Both regions consist of a main superbubble and a number of smaller, more compact HII regions that formed on the edge of the superbubble. For a total of HII regions, we systematically analyse the radiative and mechanical feedback from the massive O-type stars on the surrounding gas. We exploit the integral field property of the data and the coverage of the HeIIλ line to identify and classify the feedback-driving massive stars, and from the estimated spectral types and luminosity classes we determine the stellar radiative output in terms of the ionising photon flux Q 0 . We characterise the HII regions in terms of their sizes, morphologies, ionisation structure, luminosity and kinematics, and derive oxygen abundances via emission line ratios. We analyse the role of different stellar feedback mechanisms for each region by measuring the direct radiation pressure, the pressure of the ionised gas, and the pressure of the shock-heated winds. We find that stellar winds and ionised gas are the main drivers of HII region expansion in our sample, while the direct radiation pressure is up to three orders of magnitude lower than the other terms. We relate the total pressure to the star formation rate per unit area, Σ SFR , for each region and find that stellar feedback has a negative effect on star formation, and sets an upper limit to Σ SFR as a function of increasing pressure.