Національний фармацевтичний університет Біофармацевтичне дослідження взаємодії доксицикліну з мінеральними водами та напоями «in vitro»При одночасному прийомі антибіотиків групи тетрацикліну з компонентами їжі та напоїв може знижуватись біодоступність, ефективність препарату, змінюватись кінетика його вивільнення. Яскравим прикладом є взаємо-дія доксицикліну з антацидними препаратами, харчовими продуктами, що містять катіони лужноземельних металів, такими як молоко, сир та іншими. Проте експериментальні дані щодо можливої взаємодії доксициклі-ну з мінеральними водами та безалкогольними газованими напоями відсутні. Тому метою статті є вивчення можливої взаємодії доксицикліну з солями лужноземельних металів та її впли-ву на біодоступність в експерименті in vitro. Матеріали та методи. Об'єкти дослідження -капсули доксицикліну хіклату, мінеральні води та напої. В ході дослідження використовували аналітичні ваги Mettler Toledo AB-204/A. Тест «Розчинення» для твердих лікар-ських форм проводили на приладі PharmaTest-DT70 (Німеччина). Кількісний вміст діючої речовини визначали на спектрофотометрі «Specord 200» (Німеччина). Результати. Встановлено збільшення концентрації доксицикліну у середовищі 0,1 М розчину HCl із додаван-ням мінеральних вод: «Карпатська джерельна», «Трускавецька», «Єсентуки № 17», «Поляна квасова», а при до-даванні напою «Спрайт» спостерігається зниження концентрації порівняно із контрольним зразком. Профіль розчинення капсул доксицикліну у середовищі 0,1 М HCl із додаванням мінеральної води «Трускавецька» не є подібним у порівнянні із контрольним зразком. Профілі розчинення інших досліджуваних мінеральних вод та «Спрайту» є подібними. Висновки. Можна зробити припущення щодо можливої взаємодії катіонів кальцію та магнію, наявних у вище-зазначених водах, із молекулою доксицикліну хіклату. В практичному сенсі це означає недопустимість одно-часного прийому досліджуваних мінеральних вод із капсулами доксицикліну хіклату. Ключові слова: доксицикліну капсули; профіль розчинення; катіони металів; комплекси; взаємодія з мінераль-ними водами A. O. Dobrova, A. S Materiienko, O. S. Golovchenko, V. A. Georgiyants National University of Pharmacy The biopharmaceutical study of doxycycline interaction with mineral waters and soft drinks in vitroWhen taking the antibiotics of the tetracycline group together with food and drinks the components may decrease bioavailability, the effectiveness of the drug and change the kinetics of its release. A bright example is the interaction of doxycycline with antacid drugs, food products such as milk, cheese and others, containing alkaline earth metal cations. However, the experimental data on the possible interaction of doxycycline with mineral water and soft drinks are absent. Aim. To study the possible interaction of doxycycline with salts of alkaline earth metals and its effect on bioavailability in the experiment in vitro. Materials and methods. The study objects were doxycycline hyclate capsules, mineral waters and soft drinks. During the study a Mettler Toledo AB-204 / A a...
In recent years, the interaction of drugs with the components of food and drinks has been actively researched to ensure rational therapy. It is particularly important for amoxicillin as an antibiotic group of drugs, considering the issue of resistance. A study of the interaction of amoxicillin with mineral waters, which in significant quantities may contain cations and anions and enter complexation reactions, has not been conducted before. For the study, various chemical and physicochemical methods were used. Also in vitro dissolution test with conditions of simultaneous administration of amoxicillin with mineral waters in a 0.1M HCl was modelled. Results showed that amoxicillin could form multiple complex compounds with magnesium cations in the 0.1 M HCl with different stoichiometry. The study showed that from several investigated mineral waters, presented on the Ukrainian market, the “Karpatska dzherelna” could interact with amoxicillin in the medium of a 0.1 M HCl.
the range CZK 1,845-2,760 in the following six years. For the whole seven-year period, the total healthcare costs per person were estimated at CZK 18,223, of which CZK 9,418 were spent on pharmacotherapy. (The exchange rate fluctuated in the range 25.6-27.9 CZK/EUR in the given period.) A future increase in costs is expected in neurological care due to the risk of development of parkinsonian syndromes. Conclusions: The results of the study highlight regular stable costs related to the treatment of methanol intoxication long-term effects that must be considered for the future as well. Thus, any assessment of future healthcare costs should incorporate also long-term effects and possible complications.
The aim. To develop an EIS method for study the interaction between medicinal products and metal salts on the example of the Doxycycline and iron (III) interaction. Materials and methods. Measurements of the total impedance of the studied solutions have been performed using a vector circuit analyzer ZNB40 (Rohde & Schwarz, Germany). The calculations of electrical models were performed using the software package EC-Lab V10.40. Measurement cell was made of Teflon, 1 ml of volume, had 2 parallel nickel plated steel electrodes with diameter 6 mm, distance between electrodes is 9 mm. Basic electrical elements of model circuit were calculated according to type of electrochemical process that were described by Nyquist plot (RW, Rct, RS, Cd, CS etc.). Solutions were prepared immediately before the measurement. Measurements were performed at a temperature of 296±3 K. 6 control solutions of doxycycline and 6 control solutions of iron (III) chloride were prepared and measured. 11 study solutions at a molar ratio 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1 were prepared and measured. Concentration of the solutions was X∙10-3 mol/L respectively. Results. EIS analysis of Nyquist curves of study solutions in the range of molar ratios 1: 6, 1: 5, 1: 4, 1: 3, 1: 2, 1: 1, 2: 1, 3: 1, 4: 1, 5: 1, 6: 1 showed a change in the dynamics of electrical resistance at a ratio of 1:1. In the aqueous solution at T = 296±3 K the constant formation of the solution of doxycycline hyclate and iron (III) chloride is 2.9. This value of the complexation constant indicates that doxycycline hyclate forms a stable metal-ligand complex with iron (III) ions. Conclusions. EIS method can be applied to study the interaction of medicinal products. Model of this study was created on the example of doxycycline hyclate and iron (III) chloride. Metal-ion complexation of these two molecules was once again confirmed by using the EIS method
Aims. To study the effect of chemically modified starch D–5aM in the culture medium on the efficiency of androgenesis in vitro in anther culture of rice. Methods. Obtaining of rice double haploid lines by anther culture in vitro. The statistical methods. Results The influence different variants of gellatyne source in culture medium on the processes of induction and regeneration in anther culture of rice were studied. The 119 green plants-regenerants were received. Conclusions. The negative effect on the formation of green regenerants using a gel-forming components of the chemically modified starch D–5aM was shown. Keywords: rice, anther culture in vitro, callus, regeneration, chemically modified starch.
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