Objective: To synthesize silver nanoparticle (AgNP) using the ethanolic leaf extract of Magnolia champaca (MC-AgNP) and to evaluate its cytotoxicity and apoptotic activity in MCF-7 cell line, and to analyse active Caspase-3 expression in inducing apoptosis.
Methods: AgNP was green synthesized using ethanolic leaf extract of MC. Shape and size were analysed by SEM and particle size analyser. Cytotoxicity was tested to find IC50 value. Apoptosis was explored by mitochondrial membrane potential (ΔΨm) assay and active Caspase-3 expression study using flow cytometry.
Results: AgNP synthesized was observed to be in spherical shape through SEM micrograph with 164.6 nm in size. IC50 value of 15.90 µg/ml was obtained after 24 h of drug treatment. Changes in ΔΨm and positive active Caspase-3 protein expression were observed.
Conclusion: The present results suggest that MC-AgNP has potential anticancer property and also induced cell death via apoptosis through caspase dependent pathway in MCF-7 cells.
Heavy metals (HM) are toxic components present in the earth’s crust that can have a negative impact on plants as well as animals. Andrographis paniculata or ‘King of bitters’ belonging to the family Acanthaceae, is a medicinal herb traditionally used in the treatment of fever, common cold etc. In the present study, the effect of heavy metals (copper, tin and cobalt) on the andrographolide content, biochemical parameters like chlorophyll, carotenoid, protein, Total phenolic content (TPC), Total flavonoid content (TFC) and antioxidant activity in A. paniculata were analysed. Saplings of A. paniculata were treated at 50 and 100 mM concentrations, three different times at a time interval of 7 days. Andrographolide production was found to increase in copper and cobalt treated saplings when compared with the control. From the results, maximum andrographolide concentration was found in the saplings treated with 50 mM copper (8.51 mg/g of DW) and 50 mM tin (8.10 mg/g of DW) respectively. 50 mM cobalt treated plants have shown the highest concentration of TPC (17.21 mg/g of extract) and TFC (6.97 mg/g of extract). Notable variations in other biochemical parameters like total chlorophyll, carotenoid content and antioxidant activities were observed in all treatments compared with the control.
Andrographis paniculata is a medicinal plant that has several medicinal properties and has been
traditionally used in different medicinal preparations. The present study deals with the influence of
heavy metals (lead, mercury and silver) on andrographolide, phytochemicals and antioxidant activity
in Andrographis paniculata. Two months old saplings were subjected to heavy metal stress of two
different concentrations (0.2 mM and 0.4 mM) for three different times at 3 day time interval. The
results showed that the saplings treated with heavy metals showed increased concentration of
andrographolide content. The saplings treated with 0.4 mM silver showed the highest increase in the
andrographolide content (24.58 ± 2.85 mg/g of DW) compared with control (9.41 ± 1.26 mg/g of DW)
and other treatments. Variations in the biochemical parameters like total phenolic content, total flavonoid
content, etc. were also prominent with all the treated samples when compared to that of control.
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