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Genetic resistance to net form of net blotch in the international barley differential Canadian Lake Shore (CLS) was characterized and mapped. A doubled haploid (DH) population generated from a cross between CLS and susceptible cultivar Harrington was evaluated at the seedling stage using eight diverse Pyrenophora teres f. teres ( Ptt ) isolates and at the adult stage in the field using natural inoculum. To effectively map the CLS resistance, comparative marker frequency analysis (MFA) was performed using 8,762 polymorphic DArT-seq markers, where ‘resistant’ and ‘susceptible’ groups each comprised 40 DH lines displaying the most extreme phenotypes. Five DArTseq markers were consistently detected in eight disease assays, which was designated qPttCLS and deemed to harbor the locus underpinning CLS resistance. Four of these markers were present onto the barley DArTseq physical map and spans a region between 398203862 and 435526243 bp which were found to consist several genes involved in important plant functions such as disease response and signaling pathways. While MFA only detected the 3H region, genetic analyses based on segregation patterns were inconsistent, suggesting complex inheritance or variation in phenotypic expression of qPttCLS , particularly in the field. This study represents progress toward connecting Ptt pathotype surveys with the corresponding resistance genes in barley differentials. The markers associated with qPttCLS are useful for marker-assisted selection in breeding programs.

государственное бюджетное образовательное учреждение высшего образования «Омский государственный аграрный университет им. П.А. Столыпина», Омск, Россия 4 Федеральное государственное бюджетное научное учреждение «Всероссийский научно-исследовательский институт биологической защиты растений», Краснодар, Россия

Introduction. This study is devoted to intergroup variability of body circumferences, degree and to-pography of subcutaneous fat deposition in samples of young men of different ethnic backgrounds. Materials and Methods. We used anthropometric survey data from Russian men from various regions of Russia, indigenous Altaians and Tuvans from the Altai and Tyva Republics measured in the period from 2015 to 2019. Anthropometric measurements were performed according to standard methods of the Re-search Institute and the Museum of Anthropology of Moscow State University using GPM (DKSH, Switzer-land) and Holtain (Holtain Ltd, UK) anthropometric instruments. Body composition was assessed by bioelec-trical impedance analysis using ABC-02 Medass (Medass, Russia). We analyzed body circumferences, the value of fat and skin folds in various points of the body, bioimpedance measurements, and central fat dep-osition indices. Mathematical processing of the data was performed using the standard Statistica 10 soft-ware package. Discriminant (canonical) analysis was performed to study the topography of subcutaneous fat deposition. The materials have been collected from the population of these territories randomly and anonymously, according to the rules of bioethics and protocols of informed consent. Results. A comparative analysis of the main statistical parameters in three ethnic samples of men showed no significant differences in body mass index, the amount of fat mass, and most circumferences measurements. Intergroup variability was clearly evident in the thickness of fat and skin folds, in the index of central obesity (waist-to-height ratio), in the waist-to-hip ratio, and in the body adiposity index (BAI). In Tuvans, the value of fat and skin folds in the abdominal area and on the inner surface of the shoulder is significantly greater than in Russians and Altaians; in Russians, the mean values of all fat deposition indices are significantly lower than in Altaians and Tuvans. The canonical analysis of the set of body circumferences and fat and skin folds showed the predominant development of waist and shoulder circumferences and subcutaneous fat deposition on the body in Altaians and Tuvans as compared to Russians. Conclusions. The nature of intergroup variability indicates a predisposition of the Altaians and Tu-vans to the central type of fat deposition, which can be regarded as a result of adaptation under environ-mental stress in most Mongoloid populations of Southern Siberia.

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