One of the important directions in the field of creation and research of medicines is the optimization of the therapeutic action of the active substance and the study of the interaction of the drug and its components with the body. Numerous studies have established that a modern wound-healing agent should have the following properties: to sorb purulent discharge and its destruction products, to have cleansing properties (usually due to the introduced proteolytic enzyme), to have biocidal properties (especially with respect to pathogenic microflora and first of all with respect to to Staphylococcus aureus), because wound infection can significantly slow down the healing process, and in some cases contribute to the transition of the wound process to a chronic one and include an antioxidant (especially for the treatment of long-term diseases). The degradation of high-molecular compounds in the body can be biological and non-biological. From the point of view of the destruction of biopolymers, degradation under the influence of external factors existing in the environment of a living organism is of particular interest. The mechanism for the release of a therapeutic agent into a wound occurs due to the destruction of the base and the rupture of various links between the therapeutic agents and the matrix during its use: hydrolytic destruction under the action of the body environment, as well as biological destruction due to the action of various biomolecules in the medium, mainly enzymes. On the basis of our own and literature data, schemes have been proposed for working in a model liquid medium of wound applications, based on dialdehydecellulose and chitosan, containing immobilized therapeutic agents, including enzymes. Chitosan has been shown to stabilize dialdehyde cellulose during hydrolytic degradation. Immobilization in chitosan gel, drying and storing various therapeutic agents and their mixtures in different directions affects the kinetics of drug release.
Creating systems for targeted delivery of drugs to the affected organ is one of the most promising areas for the development of systems with controlled release of the active substance. Polysaccharides are widely used as drug carriers. However, most of them are chemically inert and require preliminary functionalization in order to interact with physiologically active compounds (therapeutic agents-TA). A simple and effective method for introducing reactive groups is the periodic oxidation of the polysaccharide by the Malaprade reaction. While cellulose is insoluble in water and resistant to weak solutions of acids and alkalis, dialdehyde cellulose (DAC is the product of the periodic oxidation of cellulose) and its derivatives are destroyed in water and weakly acidic and slightly alkaline solutions. This process is called hydrolytic destruction. The kinetics of hydrolytic destruction is described by semi-logarithmic anamorphosis, which allows us to calculate the rate constants of hydrolytic destruction as the rate constant of first-order reactions. The products of hydrolytic degradation were studied by UV spectroscopy and using 3,5-dinitrosalicylic acid (DNSA). The degradation products of C and DAC were also studied by the phenol-sulfur method. From the data presented and cited earlier, it follows that when our composite material is placed in a liquid medium, the hydrolytic destruction of the drug immediately begins. What can be connected with the breakdown of both the carrier – TA bonds (DAC, C, Ct carriers) and the destruction of the carrier itself. Under the conditions of the organism, biological destruction can also join process. Biodestruction is the process of destruction (both carriers and immobilized TAs) under the action of the body's enzymes. Using IR spectroscopy, cellulose carriers were studied before and after exposure to 1/15M FB medium (pH 6.2 and 37 °C) for 48 hours. As can be seen from the data obtained, primarily for DAC samples, significant changes in the spectrum are visible in the 1800-1600 and 900 cm-1 fields. The results of experimental toxicological studies of samples of various cellulosic materials allow us to conclude that the samples studied do not have toxic, hemolytic, allergenic effects, as well as mutagenic activity.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.