The synaptonemal complex (SC) is intimately involved in the process of meiotic recombination in most organisms, but its exact role remains enigmatic. One reason for this uncertainty is that the overall structure of the SC is evolutionarily conserved, but many SC proteins are not. Two putative SC proteins have been identified in Drosophila: C(3)G and C(2)M. Mutations in either gene cause defects in SC structure and meiotic recombination. Although neither gene is well conserved at the amino acid level, the predicted secondary structure of C(3)G is similar to that of transversefilament proteins, and C(2)M is a distantly related member of the ␣-kleisin family that includes Rec8, a meiosis-specific cohesin protein. Here, we use immunogold labeling of SCs in Drosophila ovaries to localize C(3)G and C(2)M at the EM level. We show that both C(3)G and C(2)M are components of the SC, that the orientation of C(3)G within the SC is similar to other transverse-filament proteins, and that the N terminus of C(2)M is located in the central region adjacent to the lateral elements (LEs). Based on our data and the known phenotypes of C(2)M and C(3)G mutants, we propose a model of SC structure in which C(2)M links C(3)G to the LEs. meiosis ͉ recombination ͉ chromosome ͉ immunogold ͉ electron microscopy I n general terms, the structure of the synaptonemal complex (SC) is conserved among diverse organisms with two lateral elements (LEs) that run along the length of each pair of homologous chromosomes, a central element (CE) that is located midway between the two LEs, and transverse filaments (TF) that connect the LEs to the CE (reviewed in ref. 1). However, distinct differences exist among organisms, particularly in the degree of organization of the CE (2). The conditions required for SC assembly also differ, with DNA double-strand breaks being required for SC formation in some species (e.g., budding yeast, mammals, and plants) but not in others (Drosophila and Caenorhabditis elegans) (3-5). These differences may be useful in defining nonconserved features of SC as well as in highlighting conserved functions.The morphological structures of CEs from a mammal (rat) and two insects (Drosophila and a beetle, Blaps cribrosa) were analyzed at high resolution by using EM tomography (2). In these organisms, the CE structure is essentially the same, but the degree of organization varies considerably. The CE in insects is highly organized, with two (and sometimes more) distinct longitudinal components. These dense longitudinal components appear to be composed of vertical ''pillars'' that link multiple layers of CE together. In comparison, the CE of mammals is less well organized; multiple layers of CE are not obvious, and the longitudinal components are so discontinuous that they typically appear as a single, rather broad, dark structure midway between LEs (2, 6). Some investigators have suggested that the longitudinal components are formed, at least partially, by the Nterminal domains of TFs (7, 8). Whether this difference among species in th...
Examining the relationships among DNA sequence, meiotic recombination, and chromosome structure at a genome-wide scale has been difficult because only a few markers connect genetic linkage maps with physical maps. Here, we have positioned 1195 genetically mapped expressed sequence tag (EST) markers onto the 10 pachytene chromosomes of maize by using a newly developed resource, the RN-cM map. The RN-cM map charts the distribution of crossing over in the form of recombination nodules (RNs) along synaptonemal complexes (SCs, pachytene chromosomes) and allows genetic cM distances to be converted into physical micrometer distances on chromosomes. When this conversion is made, most of the EST markers used in the study are located distally on the chromosomes in euchromatin. ESTs are significantly clustered on chromosomes, even when only euchromatic chromosomal segments are considered. Gene density and recombination rate (as measured by EST and RN frequencies, respectively) are strongly correlated. However, crossover frequencies for telomeric intervals are much higher than was expected from their EST frequencies. For pachytene chromosomes, EST density is about fourfold higher in euchromatin compared with heterochromatin, while DNA density is 1.4 times higher in heterochromatin than in euchromatin. Based on DNA density values and the fraction of pachytene chromosome length that is euchromatic, we estimate that ∼1500 Mbp of the maize genome is in euchromatin. This overview of the organization of the maize genome will be useful in examining genome and chromosome evolution in plants.[Supplemental material is available online at www.genome.org.]Maize (Zea mays ssp. mays) is both a genetic model and an economically important crop. Further progress exploiting maize as a model and a crop will be greatly aided by obtaining its complete genome sequence. However, the maize genome is large (2365 Mb vs. the 450-Mb rice genome) (Rayburn et al. 1993;Messing et al. 2004) and consists of ∼60%-80% repetitive sequence (Flavell et al. 1974;Hake and Walbot 1980;Messing et al. 2004). Both of these features make sequencing and assembling the entire maize genome difficult. Methods that concentrate on sequencing the gene space promise to provide important information on genetically active regions of the maize genome (Palmer et al. 2003;Whitelaw et al. 2003;Yuan et al. 2003) but, by their very nature, will not elucidate large-scale genome organization.To date, several strategies have been used to provide insights into maize genome organization. These include the use of highdensity genetic linkage maps (Davis et al. 1999;Cone et al. 2002;Sharopova et al. 2002), methylation filtration and high C o t selection to sequence the gene space (Palmer et al. 2003;Whitelaw et al. 2003;Yuan et al. 2003), and DNA contigs constructed from overlapping BACs (Coe et al. 2002;Cone et al. 2002). Many of these BAC contigs have been anchored by genetic markers to a commonly used linkage map (IBM2 Neighbors; http:// www.maizegdb.org) and are useful both for estimatin...
Recombination nodules (RNs) are closely correlated with crossing over, and, because they are observed by electron microscopy of synaptonemal complexes (SCs) in extended pachytene chromosomes, RNs provide the highest-resolution cytological marker currently available for defining the frequency and distribution of crossovers along the length of chromosomes. Using the maize inbred line KYS, we prepared an SC karyotype in which each SC was identified by relative length and arm ratio and related to the proper linkage group using inversion heterozygotes. We mapped 4267 RNs on 2080 identified SCs to produce high-resolution maps of RN frequency and distribution on each bivalent. RN frequencies are closely correlated with both chiasma frequencies and SC length. The total length of the RN recombination map is about twofold shorter than that of most maize linkage maps, but there is good correspondence between the relative lengths of the different maps when individual bivalents are considered. Each bivalent has a unique distribution of crossing over, but all bivalents share a high frequency of distal RNs and a severe reduction of RNs at and near kinetochores. The frequency of RNs at knobs is either similar to or higher than the average frequency of RNs along the SCs. These RN maps represent an independent measure of crossing over along maize bivalents.
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