Aspidistra guizhouensis S.Z.He & W.F.Xu, a new species of Aspidistra from Guizhou province, China, is described, illustrated and a line drawing of the species is provided. Aspidistra guizhouensis is similar to the widespread species Aspidistra retusa K.Y.Lang & S.Z.Huang and Aspidistra sichuanensis K.Y.Lang & Z.Y.Zhu. The karyotype of this species is analysed.
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