In the Golli-tau-eGFP (GTE) transgenic mouse the reporter gene expression is largely confined to the layer of subplate neurons (SPn), providing an opportunity to study their intracortical and extracortical projections. In this study, we examined the thalamic afferents and layer IV neuron patterning in relation to the SPn neurites in the developing barrel cortex in GTE mouse at ages embryonic day 17 (E17) to postnatal day 14 (P14). Serotonin transporter immunohistochemistry or cytochrome oxydase histochemistry was used to reveal thalamic afferent patterning. Bizbenzimide staining identified the developing cytoarchitecture in coronal and tangential sections of GTE brains. Enhanced green fluorescent protein (GFP)-labelled neurites and thalamic afferents were both initially diffusely present in layer IV but by P4-P6 both assumed the characteristic periphery-related pattern and became restricted to the barrel hollows. This pattern gradually changed and by P10 the GFP-labelled neurites largely accumulated at the layer IV-V boundary within the barrel septa whereas thalamic afferents remained in the hollows. To investigate whether this reorganisation is dependent on sensory input, the whiskers of row 'a' or 'c' were removed at P0 or P5 and the organisation of GFP-labelled neurites in the barrel cortex was examined at P6 or P10. In the contralateral region corresponding to row 'a' or 'c' the lack of hollow to septa rearrangement of the GFP-labelled neurites was observed after P0 row removal at P10 but not at P6. Our findings suggest a dynamic, sensory periphery-dependent integration of SPn neurites into the primary somatosensory cortex during the period of barrel formation.

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i i 818 7QH B r l y m a d The dationship k(wcen hormone rep-h p y (HRV with mmIy bean disease (CHD) and hyperlension (HT) is still not welluadastood Reviousobservational studiessuggestinga 5O%cardiovamdarrisk ductionare not supponedby recenl ratdomised trials However, HRT remains usefol for amlioration dmmpausal symP(0ms and prevention of we invesligaced into the prescribiag wits in pnrclice and w s and €IT are important Mom detamining HRTuse. Mclbodh Data from I 4 0 wmen already on HRT were a w l l e d with 140 --usem from a single general practice. Bloodpresgure (BP) was noted at start and I ycar. 8. HRTUsrsNon-Usas Pvaluc) DM 3.5% 8.6% NS CHD 2.1% 5.Wh NS HT 20.Wh 18.6% NS Systolic BP(mmiHg): MeanBaselinc(SD) 129.8 (18.2) 136.4 (18.1) 0.0024 Meanductionat lycar 3.28. N5 *confidence interval 0.1254.446(p=0.042)R e u l h T b e r e w e r e n o d i f f~b e ( w e e n u s e m a n d n o nforbaseline cdiovascular risk faclm: smoking, disbe(es mllitus (DM), known CHD and HT (systolic BP >I.00mmHG and/or diastolic BP W). The main indication for HRTusewasmwpausalsympoms(71.4%ofusem)followedbypreventionof -(II.~%O~IICMI-WWS). 85.7Y.ofO00-didnotha~a11yknown~~-indications. Rimary and serxlndary Cardiovascular prevention did *,figure a contramdm tion among 0 0 0 -. Mean duration of HRTuse was 5.3 years, with 8.5% on HRT for 10 years or mre. Baseline systolic BP was significantly lower among HRT usen and fell further dwing HRT use. Conclusion CHD and risk profile is not mutineb considmd when 8hcatin.g or precludine the use of HRT. This may be due to e0oflidin.g e v i b m Pertaining the safely and elficacy of HRT use in womea. Therewasnoadverscrelalionshipbe(ween HRTandbloodprssure. oslmporosis (7.1%). The main co-cation among mn-usem was breaa among the indications for HRT use nor did CHD present p w m d CHD but msy also be due to 1 -impoaance ofcHD attached to 33 EFFECTS OF CARVEDILOL, A VASODILATORY BETA-BLOCKER, ON MlCROALBUMINURlA IN HEART FAILURE B CHIN, R HAYNES, M LAL and GYH LIP* Universityt of Medicine, City Hospital, Dudley Road Birmingham B18 7QH Background Micdbuminuria (defined as urinary albumin excretion

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