Arbuscular Mycorrhyzae Fungal (AMF) spores were obtained through mass multiplication in maize (in a previous experiment) as a host crop. A study was conducted to test the effectiveness of AMF in rice as host, with three levels of P 2 O 5 (0, 20 and 40 kg/ha) under submerged condition. By reducing levels of P 2 O 5 to 50 % (20 kg/ha) comparable plant biomasses were recorded (P < 0.05) under AMF inoculation with that of highest level of P 2 O 5 (40 kg/ha) with or without AMF inoculation. By reducing the P supply (20 kg/ha), measurable effect of mycorrhyzae (P < 0.05) was obtained for P and N concentration (1.27 and 7.88mg/g respectively) and their uptake (20.63 and 127.50 mg/plant respectively) in rice plants which were comparable with un-inoculated plants at 40 kg P 2 O 5 /ha. AMF spores and root colonization were found at all levels of P supply, although overall the numbers of mycorrhyzal spores and per cent root colonization decreased with increasing P under mycorrhyza inoculation which implies that saturation or inundation does not necessarily prevent the development of AMF association.
Sugarcane plants including roots were collected from 21 sugarcane growing locations of Golaghat, Dimapur and Jorhat districts of North Eastern region for isolation of endophytic Gluconacetobacter. Based on the nitrogenase activity, only eight isolates were selected for further characterization of Gluconacetobacter. The nitrogenase activity of the isolates ranged in between 12.4 to 72.4 n mole C2H4 h-1 culture-1. The characteristic yellowish surface pellicles were formed on inoculating the selected isolates into semisolid LGI medium and acetic LGI medium. On enumeration in LGI plates, the frequencies of well recognized Gluconacetobacter like colonies ranged in between 0.75 and 7.68 x 105 (cfu g-1 fresh tissue). Colony morphology of the isolates was irregular flat, irregular raised or circular raised yellow colonies of 1-5 mm in diameter. Light brown to brown pigmentation was observed on potato agar plates and light brown pigmentation with clearing of plates was observed on GYC plates. All the isolates exhibited gram negative and shape of the cells were rods. Reactions to extracellular and intracellular enzymes as well as to the various carbon sources by the isolates explained the diversity among them. The IAR profiles of the eight isolates against twelve antibiotics exhibited wide variation in their response to various antibiotics.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.