Material and methods Cloning of a full-length eDNA for hCyP33PCR amplification of DNA-fragments from different human Jurkat T cell line cDNA libraries were performed with two degenerate primers, which correspond to amino acid sequences QGGDF and KHVVFG (boxed in Fig. 2b) in the highly conserved regions of known cyclophilins [3,13,[17][18][19][20]. The resulting PCR products were subcloned into pUC18 and sequenced. Nested PCR was performed to extend the sequence using two libraries: a Marathon cDNA library of the human Jurkat T cell line (Clontech) and a cDNA library made of mRNA from human T cells. Both libraries yielded as longest cDNA one with a length of 1.6 kb. The eDNA was sequenced on both strands.

No abstract