The purified fish visceral protease enzyme was immobilized by using various concentrations of sodium alginate and calcium chloride to optimize the best concentration for the formation of the beads. Then it was characterized by assaying the optimal pH, temperature, storage stability and reusability. The results on immobilization with sodium alginate and calcium chloride showed that a combination of 2% sodium alginate and 0.3 M calcium chloride weas found to be the optimum concentration for the formation of spherical and stable beads, this gave a maximal entrapped activity of 48.31%, and there was no change in the optimum pH 8.0 and temperature 40°C of protease before and after entrapment. The results on stability and reusability indicated that it was stable at 4°C retaining 100% residual activity after 5 days of storage and 67% loss of activity after ten days of storage and it retained 100% residual activity on the first reuse, 75% residual activity on the second reuse, 25% residual activity on the third use and complete loss in the activity on the fourth reuse.
Nanoparticles are widely used in the biotechnology and biomedicalfield. Green synthetic methods of nanoparticles are a simple and environmentallybenign process that declines the demerits of conventional chemical andphysical methods. The synthesis of semiconductor and metal nanoparticles is a risingdo research part, due to the possible applications in the progress of new technologies.The present research information the synthesis and characterization of zinc oxide nanoparticles (ZnO NPs) using C. Sinensis leaf extract. The findings of these studies show the green synthesized ZnO NPs are effective, safe, and eco-friendly as they arestable and have abundant flower shapes with maximum particles in size ranging from 100 nm indiameter. The synthesized ZnO NPs have been tested against the pathogenic microorganismsand showed an excellent zone of inhibition. DPPH radical scavenging activity of synthesized ZnO NPs expressed DPPH free radicals as a percentage of inhibition andIC50value of 70.37%. Acetylcholine esterase (AChE) inhibitory activity possesses the maximum amount of the synthesized ZnO NPs. Since the results ofthe current work, it was concluded that the synthesized ZnO NPs exhibited significant antibacterial and acetylcholine esterase inhibitory activities. Hence it can beused as a drug with multifunction in treating Alzheimer’s disease (AD).
Alzheimer’s disease is a progressive neurodegenerative disorder with neuropsychiatric symptoms and several cognitive functions and is biochemically characterized by a significant decrease in the brain neurotransmitter acetylcholine. The current study is performed to investigate possible interaction of the active components identified from Camellia sinensis (Green tea) with acetyl cholinesterase (AChE) through docking studies using Schrödinger software (Maestro V: 11.8 Schrödinger_suite-2019). In silico study results clearly showed that catechin and epicatechin-3-gallate binds effectively with AChE through strong hydrogen bonding
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