Introduction and Aim: Owing to its medicinal properties, in the current investigation, crude extracts and liquid fractions of stem bark of Rhus mysorensis (RM) were screened for phytochemical composition and in vitro antiproliferative activity. Materials and Methods: Phytoconstituents in extracts were examined using standard qualitative tests. TFC and TPC of the extracts were measured using the aluminum chloride and the Folin-Ciocalteau methods respectively. In vitro antiproliferative activity of obtained five crude extracts (RMHE, RMPE, RMCE, RMME and RMAE) and three liquid fractions (RMLH, RMLC and RMLM) were determined in MDA-MB-231 cells by MTT assay. Results: Different types of phytoconstituents were detected in crude extracts. The total phenolic contents of RMME (295.92 ± 0.32 mg Gallic acid/g extract) while total flavonoids contents of RMHE (75.0 ± 4.33mg Catechin / g of extract) were found significantly higher as compared to other solvent extracts. RMME exhibited the highest antiproliferative activity on MDA-MB-231 cells followed by RMCE and RMHE with IC50 values of 79.60 ±1.05, 86.81±1.11 and 121.45 ± 6.03?g/mL respectively. RMPE and RMAE did not show significant growth inhibition. Unlike other crude extracts, RMME did not inhibit the growth of L6 cells even at higher concentration (320?g/mL). Among the solvent fractions, RMLM demonstrated potent antiproliferative activity in MDA-MB-231 (IC50, 60.85±1.59?g/mL) cells while RMLH and RMLC showed comparatively lesser growth inhibitory effect with IC50 values of 111.3 ± 0.35 and 124.1 ± 2.74?g/mL respectively. Conclusion: The extracts of Rhus mysorensis were shown to contain a remarkable amount of different bioactive compounds, thus confirming their involvement in several biological activities. RMLM selectively inhibit the proliferation of TNBC (triple negative breast cancer) cells hence it can be used as a source for isolation and characterization of novel anticancer compounds.
Since ancient times human beings are using plant-based medicines for the treatment of various ailments, especially in the rural areas, due to their availability and affordability. Rhus mysorensis (RM) is widely used as a traditional medicine to treat various ailments. Owing to its potential medicinal value, the present study was designed to explore the in vitro antioxidant, anti-inflammatory, anticoagulant and antiplatelet properties of purified column fraction of RM. The methanol extract of stem bark powder was sequentially fractioned by solvent partitioning. The liquid methanol fraction was further fractionated by column chromatography using gradient elution. Eluted fractions were analyzed using HPLC for percentage purity and yield. The fraction with higher percentage of purity and yield was assessed for in vitro antioxidant activity by measuring SOD and GPx activities, anti-inflammatory activity by the inhibition of nitric oxide (NO) production in LPS induced RAW264.7 cells, anticoagulation by plasma recalcification time and antiplatelet activity by agonists induced platelet aggregation respectively. The antioxidant potency of column fraction (B8) revealed that, highest enzyme activities were recorded at a concentration of 320µg/ml. The enzyme activity was found to be 2.45 U/ml for SOD and 135.75 U/L for GPx respectively. Purified column fraction (B8) of RM significantly reduces the production of NO in LPS stimulated RAW 264.7 cell lines at 320????g/ml concentration with 31.90% of inhibition. The anticoagulant activity of purified fraction was determined in terms of plasma recalcification time. Interestingly, the fraction showed the most potent anticoagulant activity both in PRP and PPP as it prolonged the clotting time. The findings indicate that the stem bark of RM possesses potent antioxidant, anti-inflammatory, anticoagulant and antiplatelet activities, supporting the use of this species for treating oxidative stress-induced inflammatory diseases. Further, bioactivity guided fractionation studies to characterize and identify specific phytochemicals responsible for these biological activities are needed.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.