Piper zeylanicum Miq. (Piperaceae) an endemic species of Sri Lanka is reported and described and illustrated as a new record for India from Nilgiri District of Tamil Nadu.
The present study is an attempt to evaluate the pharmacognostic parameters and HPTLC fingerprint profiles for a Siddha compound formulation, Pirantai vatakam in which Cissus quadrangularis is the main ingredient. Powder microscopy studies were carried out and different microscopic characters were distinguished. Physico-chemical parameters such as loss on drying at 105⁰C, total ash, acid insoluble ash, water soluble extractive, alcohol soluble extractive AND volatile oil were determined. High performance thin layer chromatographic (HPTLC) study of Pirantai vatakam was performed and the chromatograms were documented. The observations laid down a platform for the standardization of Pirantai vatakam and will help us to determine the genuineness of the drug from the adulterants and substitutes.
The present study attempts to standardize the pharmacognostic, physic-chemical parameters, UV-Vis and HPTLC fingerprinting of the plant, Ruellia tuberosa. Various trichome morphotypes were the unique observation seen in the plant by anatomical as well as powder microscopic studies. Four prominent peaks were detected by UV-Visible spectroscopy and HPTLC fingerprint revealed many peaks with a wide range of Rf values. The present study of botanical and chemical screening will be useful for developing pharmacopeial standards for R. tuberosa.
Objective: In this study, an attempt was made to generate information based on botanical, physicochemical and HPTLC data needed for proper identification and authentication of M. emarginata and C. asiatica belonging to two different families.
Methods: Botanical study comprises of macroscopy, microscopy and powder microscopy of leaves of both crude drugs. The physicochemical parameters such as water-soluble extractive, alcohol soluble extractive and loss on drying at 105℃, total ash, acid insoluble ash, and volatile oil were determined according to standard methods. HPTLC studies of chloroform extracts of leaves of both drugs were conducted at 254 nm, 366 nm and 575 nm after derivatisation using vanillin-sulphuric acid and the results were documented.
Results: The present study reveals that microscopy and most of the physicochemical parameters of both the plant materials are different. Anatomy of the leaves showed two main characteristic differences. First plenty of trichome with trichome base and calcium oxalate crystal is common in M. emarginata, which is not observed in C. asiatica. Both plants have different venation patterns and leaf constants. The total ash content and the solubility in alcohol and water for leaves of C. asiatica are higher than that of M. emarginata. The HPTLC fingerprinting pattern obtained for both drugs are different.
Conclusion: All the results obtained from this study help in determining differences and similarities of leaves of M. emarginata and C. asiatica and thereby preventing adulteration and substitution and emphasizing the importance of standardization.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.