The purpose of this study was to determine the quality of the ability of spermatozoa cells to maintain the use of a yolk citrate (YC) diluent with the addition of palmyra palm juice during preservation. The material used for this research was 2 Sumba Ongole bulls as a source of semen. Semen was collected by using an artificial vagina that is processed twice a week. The obtained semen was macroscopically analyzed, including volume, colour, consistency, pH and smell. Meanwhile, microscopic evaluation includes mass movement, motility, concentration, viability and abnormalities. The semen showed >75% motile sperm divided into four treatments and dilute each with YC (P0), 95% YC+5% palmyra palm juice (PPJ) (P1), 90% YC+10% PPJ (P2), 85% YC+15% PPJ and each was stored at 3-5 0 C. The results of statistical analysis showed that P0, P1 treatments were significantly different (P<0.05) between P2, P3 from day 0 to day 4 of storage. The addition of palmyra palm did not affect the motility of spermatozoa, but had a positive effect on the viability of spermatozoa with the addition of 5% palm sap during storage for 4 days at a temperature of 3-5 0 C.
Penelitian ini bertujuan untuk mengetahui kualitas spermatozoa dalam pengencer tris kuning telur dengan penambahan level nira lontar yang berbeda. Materi penelitian ini menggunakan 2 ekor sapi Sumba Ongole sebagai sumber semen. Penampungan semen menggunakan vagina buatan yang ditampung 2 kali dalam seminggu. Semen yang diperoleh dilakukan evaluasi secara makroskopis yang meliputi volume, warna, konsistensi, pH dan bau. Sedangkan evaluasi mikroskopis meliputi gerakkan massa, gerakan individu, konsentrasi, viabilitas dan abnormalitas. Sperma menunjukkan >75% sperma motil dibagi menjadi empat perlakuan dengan masing-masing pengencer yaitu: P0 = Pengencer tris kuning telur (TKT) 100%, P1 = pengencer TKT 95% + nira lontar 5%, P2 = pengencer TKT 90% + nira lontar 10%, P3 = pengencer TKT + nira lontar 15%. Hasil analisis of variance menunjukkan bahwa perlakuan P2 berbeda nyata (P<0,05) antara P1, P2, P3 terhadap motilitas spermatozoa dan viabilitas sapi Sumba Ongole. Dengan demikian dapat disimpulkan bahwa penambahan nira lontar berpengaruh pada motilitas spermatozoa dengan level nira lontar terbaik 10%.
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