Nitrate reductase (NADH: nitrate oxidoreductase, EC 1.6.6.1) of spinach (Spinacia oleracea L.) leaves, inactivated in vitro by acetylene, was reactivated by irradiation with blue light. Red + infrared, green or white light of the same irradiance were less effective. The dehydrogenase activity of the nitrate reductase complex was not required for pliotoreactivation. Photoreactivation of cyanide‐inactivated nitrate reductase was greatly enhanced by the addition of 1 and 20 μ of either FMN or FAD; however, flavins showed a much smaller stimulatory effect on photoreactivation of acetylene‐inactivated enzyme. The effect of flavins was higher under anaerobic conditions. This might imply the direct ievolvement of excited flavins in the photoreactivation mechanism. Besides promoting photoreactivation, blue light irradiation led simultaneously to a gradual inactivation of the enzyme especially under air and 20 μ FMN, eventually abolishing the recovered activity of the enzyme.
La pu bli ca ción elec tró ni ca: ¿Un pa ra dig ma de or ga ni za ción do cu men tal di gi tal? RE SU MENLa pu bli ca ción elec tró ni ca se en cuen tra es tre cha men te re la cio na da con la tec no lo gía que la hace po si ble, y ha in tro du ci do mo di fi ca cio nes en los proce sos de edi ción de do cu men tos, dan do lu gar a la crea ción de nue vas formas, pro ce sos y me dios de pu bli ca ción que con du cen a la crea ción de un pa ra dig ma de or ga ni za ción do cu men tal di gi tal. El len gua je uti li za do para alu dir a la pu bli ca ción elec tró ni ca de ri va tan to del cam po de las tec no lo gías de la in for ma ción, como del uti li za do por las em pre sas de di ca das a la pro duc ción de soft ware para pu bli ca ción en lí nea y en cd-rom. Has ta el mo men to no exis te en la li te ra tu ra una cla ri dad sobre los con cep tos re la cio na dos con este fe nó me no. Por ello en el pre sente tra ba jo se iden ti fi can, sis te ma ti zan y es truc tu ran aque llos ele men tos que se con si de ra pue den per mi tir con cep tuar el fe nó me no de la pu bli cación elec tró ni ca ELEC TRO NIC PU BLISHING: A PA RA DIGM FOR DI GI TAL DO CU MENT ORGANIZATION?RAMI RO LAFUEN TE-LÓPEZ ANGÉ LI CA MARÍA ROSAS-GUTIÉ RREZ ABSTRACT Elec tro nic pu blishing is clo sely re la ted to the te chno logy that ma kes it pos si ble. Con se quently, it is a phe no me non which po ses for the elec tronic pu blishing en vi ron ment in par ti cu lar, and for pu blishing in ge ne ral, mo di fi ca tions to the edi to rial pro cess, es pe cia lly with res pect to the concep tion of the idea of a pu blisha ble pro duct, as well chan ges in the concepts of pu bli ca tion, sin ce in many ca ses it re fers to an upda ting type of ser vi ce. The ge ne ra tion of the elec tro nic pu bli ca tion thus re qui res the use of concepts de ri ved both from the use of such te chno logy and from the ma nufac tu rers of soft ware for the pu blishing in dus try. Up to the pre sent time the re has been a lack of cla rity and hie rar chy in the li te ra tu re in res pect to the con cepts re la ted to this phe no me non. It is for this rea son that the pre sent study iden ti fies, clas si fies, and struc tu res the ele ments that concep tua li ze the elec tro nic pu bli ca tion. IN TRO DUC CIÓNE l fe nó m e n o de la pu bli ca ción elec tró ni ca está es tre cha men te aso cia do a la so cia li zación del uso de las com pu ta do ras, de las tec no lo gías de la in for ma ción y al cre cimien to de las re des de te le co mu ni ca ción para trans mi tir in for ma ción. Como no po demos se pa rar lo de las tec no lo gías que la ha cen po si ble, pues to que és tas de ter mi nan el per fil de ma nu fac tu ra de los do cu men tos di gi ta les, es in dis pen sa ble iden ti fi car al gu nas de las ten den cias pre do mi nan tes en cuan to a las tec no lo gías apli ca das al di se ño, produc ción y di fu sión de do cu men tos di gi ta les, a par tir de las cua les se pue dan in fe rir las ca rac te rís ti cas de esta nue va for ma de or ga ni za c...
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