The dominant process adding nitrogen (N) to the ocean, di-nitrogen (N2) fixation, is mediated by prokaryotes (diazotrophs) sensitive to a variety of environmental factors. In particular, it is often assumed that consequential rates of marine N2 fixation do not occur where concentrations of nitrate (NO−3) and/or ammonium (NH+4) exceed 1μM because of the additional energetic cost associated with assimilating N2 gas relative to NO−3 or NH+4. However, an examination of culturing studies and in situ N2 fixation rate measurements from marine euphotic, mesopelagic, and benthic environments indicates that while elevated concentrations of NO−3 and/or NH+4 can depress N2 fixation rates, the process can continue at substantial rates in the presence of as much as 30μM NO−3 and/or 200μM NH+4. These findings challenge expectations of the degree to which inorganic N inhibits this process. The high rates of N2 fixation measured in some benthic environments suggest that certain benthic diazotrophs may be less sensitive to prolonged exposure to NO−3 and/or NH+4 than cyanobacterial diazotrophs. Additionally, recent work indicates that cyanobacterial diazotrophs may have mechanisms for mitigating NO−3 inhibition of N2 fixation. In particular, it has been recently shown that increasing phosphorus (P) availability increases diazotroph abundance, thus compensating for lower per-cell rates of N2 fixation that result from NO−3 inhibition. Consequently, low ambient surface ocean N:P ratios such as those generated by the increasing rates of N loss thought to occur during the last glacial to interglacial transition may create conditions favorable for N2 fixation and thus help to stabilize the marine N inventory on relevant time scales. These findings suggest that restricting measurements of marine N2 fixation to oligotrophic surface waters may underestimate global rates of this process and contribute to uncertainties in the marine N budget.
. One important caveat is that fixed N in atmospheric deposition may contribute a significant proportion of the low-d 15 N N in the Sargasso Sea thermocline, in which case the relatively low rate we estimate for N 2 fixation would still be too high.
[1] Below its sill depth, the Santa Barbara Basin (SBB) is commonly suboxic ([O 2 ] $ 3 mM), with only brief periods of ventilation. Associated with development of suboxia, the concentration of nitrate decreases with depth into the basin without an associated decrease in phosphate, indicating that a substantial fraction of the nitrate supplied to the basin is removed by denitrification. Coincident with the decrease in nitrate concentration across the ''redoxcline'' (the interface between oxic and suboxic waters) within the SBB, there is an increase in the 15 N/ 14 N of that nitrate, as would be anticipated from the isotopic fractionation associated with denitrification. However, the increase in O suggest that the lowerthan-expected 15 N enrichment in the suboxic SBB involves denitrification, rather than being due to some unknown source of low-15 N/ 14 N N to the deep SBB. Calculations with a range of models of nitrate supply and consumption indicate that the degree of nitrate consumption in the basin is too small for differences in water circulation to explain the isotopic differences between the Santa Barbara Basin and the open ETNP. Previous studies indicate that the isotope effect of sedimentary denitrification is negligible due to nitrate diffusion in sediment pore waters. Thus we infer that the small magnitude of the isotopic enrichment of SBB water column nitrate is due to the importance of sedimentary denitrification within the basin. Assuming that water column and sedimentary denitrification have isotope effects of 25 and 1.5 per mil, respectively, our results suggest that sedimentary denitrification accounts for more than 75% of the nitrate loss within the suboxic SBB.
The allochthonous supply of dissolved organic nitrogen (DON) from gyre margins into the interior of the ocean's oligotrophic subtropical gyres potentially provides an important source of new N to gyre surface waters, thus sustaining export production. This process requires that a fraction of the transported DON be available to euphotic zone photoautotroph communities via mineralization. In this study, we investigated the biological and physical controls on the distribution and fate of DON within global ocean surface waters. Inputs of nitrate to the euphotic zone at upwelling zones fuel net accumulation of a DON pool that appears to resist rapid microbial remineralization, allowing subsequent advective transport into the subtropical gyres. Zonal gradients in DON concentrations across these gyres imply a DON sink in the surface layer. Assessment of the physical dynamics of gyre circulation and winter mixing revealed a pathway for DON removal from the mixed layer via vertical transport to the deep euphotic zone, which establishes the observed zonal gradients. Incubation experiments from the Florida Straits indicated surface‐accumulated DON was largely resistant (over a few months) to utilization by the extant surface bacterioplankton community. In contrast, this same material was remineralized three times more rapidly when exposed to upper mesopelagic bacterioplankton. These results suggest the primary fate of surface DON to be removal via vertical mixing and subsequent mineralization below the mixed layer, implying a limited role for direct DON support of gyre export production from the surface layer. DON may contribute to export production at the eastern edges of the subtropical gyres, but only after its mineralization within the deep euphotic zone.
An extensive region of the Eastern Tropical South Pacific (ETSP) Ocean has surface waters that are nitrate-poor yet phosphate-rich. It has been proposed that this distribution of surface nutrients provides a geochemical niche favorable for N 2 fixation, the primary source of nitrogen to the ocean. Here, we present results from two cruises to the ETSP where rates of N 2 fixation and its contribution to export production were determined with a suite of geochemical and biological measurements. N 2 fixation was only detectable using nitrogen isotopic mass balances at two of six stations, and rates ranged from 0 to 23 μmol N m −2 d −1 based on sediment trap fluxes. Whereas the fractional importance of N 2 fixation did not change, the N 2 -fixation rates at these two stations were several-fold higher when scaled to other productivity metrics. Regardless of the choice of productivity metric these N 2 -fixation rates are low compared with other oligotrophic locations, and the nitrogen isotope budgets indicate that N 2 fixation supports no more than 20% of export production regionally. Although euphotic zone-integrated short-term N 2 -fixation rates were higher, up to 100 μmol N m, and detected N 2 fixation at all six stations, studies of nitrogenase gene abundance and expression from the same cruises align with the geochemical data and together indicate that N 2 fixation is a minor source of new nitrogen to surface waters of the ETSP. This finding is consistent with the hypothesis that, despite a relative abundance of phosphate, iron may limit N 2 fixation in the ETSP.nitrogen fixation | eastern tropical south Pacific | nitrogen budgets | nitrate | nitrogen isotopes
Measurements to date have shown that both bulk and high molecular weight marine dissolved organic nitrogen (DON) have a 15N/14N that is substantially higher than the 15N/14N of suspended particulate organic nitrogen (PNsusp) found in the same surface waters (with δ15N of ∼4 to 5‰ and ∼−1 to 1‰, respectively). Moreover, the concentration and 15N/14N of DON are much less dynamic than those of PNsusp. These observations raise questions regarding the role of DON in the upper ocean nitrogen (N) cycle. In this study, the concentration and 15N/14N of nitrate and DON was measured in the upper 300 m of the oligotrophic North Atlantic and North Pacific Oceans. Comparing these two regions, the average DON concentration in the upper 100 m is similar, between 4.5 and 5.0 μM, but the average δ15N of DON is significantly different, 3.9‰ versus air in the North Atlantic and 4.7‰ in the North Pacific. This difference parallels a similar isotopic difference between shallow nitrate in these two regions; at 200 m in the North Atlantic, the δ15N of nitrate is 2.6‰, while it is 4.0‰ in the North Pacific. This isotopic correlation between surface DON and subsurface nitrate indicates that DON is actively participating in the upper ocean N cycle of each region. We describe a conceptual model that explains the elevation of the 15N/14N of DON relative to surface ocean PNsusp as well as the interbasin difference in the 15N/14N of DON. In this model, DON is produced from PNsusp without isotopic fractionation but DON is removed by fractionating processes. The ammonium and simple organic N compounds released by DON decomposition reactions are reassimilated by algae into the PNsusp pool, as an integral part of the ammonium‐centered cycle that lowers the 15N/14N of PNsusp relative to the nitrate supply from below. This interpretation is consistent with the understanding of the chemical controls on isotope fractionation and is analogous to the previously posed explanation for the 15N/14N elevation of herbivorous zooplankton. In addition, it explains a lack of correlation between in situ N2 fixation rates and DON concentration and 15N/14N on short time scales.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.