Effect of sugarcane molasses on the in vitro development of banana (Musa spp.) cv. MaçãDespite offering a number of advantages as the quality of final product, the technique of in vitro cultivation of plants is still considered expensive due to, among other things, the use of reagents with high purity for the preparation of nutrient media. Among the alternatives that can be adopted to reduce the costs associated with the production of seedlings, the replacement of the PA product for those with lower has potential. Based on this information, the purpose of this study was to evaluate the effect of molasses of sugar cane on in vitro development of banana cv. Maçã. For this purpose, banana plants were inoculated in nutrient media made on the basis of sugarcane molasses, with four different concentrations (Brix of 1.5, 3.0, 4.5 and 6.0) and data were compared with those of plants grown on MS medium (control), in a total of five treatments. Despite plants have been developed in all treatments, it was observed that for the variables leaf number and fresh biomass, plants cultivated in MS medium (control) had higher performances. However, for the average number of roots, there was no statistical difference between treatments. The sugarcane molasses did not favor the development of banana plants, but can be used for in vitro rooting of these plants. Apesar de oferecer uma série de vantagens, quanto à qualidade do produto final, a técnica de cultivo in vitro de plantas ainda é considerada dispendiosa, por causa, dentre outros fatores, da utilização de reagentes com alto grau de pureza no preparo de meios nutritivos. Entre as alternativas que podem ser adotadas para a redução dos custos relacionados com a produção de mudas, apresenta potencial a substituição de produtos PA por aqueles de menor custo. Baseado nestas informações, o objetivo deste estudo foi avaliar o efeito do melado de cana-de-açúcar sobre o desenvolvimento in vitro de bananeira, cv. Maçã. Para tal propósito, plantas de bananeira foram inoculadas em meios nutritivos, formulados à base de melado de cana-de-açúcar, com quatro concentrações distintas (brix de 1,5; 3,0; 4,5 e 6,0), e os dados obtidos foram comparados com aqueles das plantas cultivadas em meio MS (controle), perfazendo um total de cinco tratamentos. Apesar de terem-se desenvolvido em todos os tratamentos testados, observou-se que, para as variáveis número médio de folhas e biomassa da matéria fresca, as plantas cultivadas no meio MS (controle) apresentaram valores superiores. Entretanto, para o número médio de raízes, não houve diferença estatística entre os tratamentos utilizados. O melado de cana-de-açúcar não favorece o desenvolvimento de plântulas de bananeira, mas pode ser utilizado para o enraizamento dessas plantas in vitro.Palavras-chave: redução de custos, micropropagação, reagente comercial.
Use of panela as nutritive medium for in vitro culture of banana cv. MaçãThe purpose of this study was to evaluate the effect of panela (hard brown sugar) on in vitro development of banana cv. Maçã, in order to reduce production costs. Banana explants were inoculated in nutrient media formulated with four different concentrations of panela solution (10, 25, 50 and 75%) and data were compared with those of plants grown on MS medium (control), totalizing five treatments. After 60 days, the average number of leaves, shoots and roots, as well as the mean number of explants dead and oxidized were analyzed. There was no significant difference between treatments for most parameters, except for the mean number of oxidized explants, which was higher in the treatment containing 75% of panela. It was concluded that nutrient media with up to 50% of panela in the composition, without plant growth regulators, can be used for in vitro banana replacing the MS media.
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