SUMMARYSalmonellosis remains an important cause of diarrheal illness in humans in São Paulo State, Brazil. In this study were identified 3554 Salmonella isolates from human infections, during the period 1996-2003. Among 68 different serovars determined, S. Enteritidis was the most frequent one in gastrointestinal and extra-intestinal infections accounting for 67.4% of all isolates. S. Typhimurium and S. enterica subsp. enterica (4,5,12:i:-) were most frequently isolated from children aged < 1-4 year-old, in contrast, people with S. Enteritidis infections were most likely to be 20-50 year-old. In our geographic area the continued laboratorial surveillance of salmonellosis, including serotyping, has showed the trends in Salmonella serovars causing infections in humans throughout the time.
A total of 283 Salmonella Typhimurium strains isolated from cases of human infections and non human sources, were examined for antimicrobial susceptibility and the incidence of resistance was 38% and multiple resistance (to three or more antimicrobials) was 15%. All 43 multidrug-resistant strains (MDR) and 13 susceptible ones were characterized by phage typing and pulsed-field gel electrophoresis (PFGE). The strains encompassed 14 definitive phage types (DT), three were untypable (UT), and 18 atypicals or reaction does not conform (RDNC), which belonged to 21 PFGE patterns, A1-A21. The predominant phage types were DT49, DT193, and RDNC and two strains belonging to DT 104 and 104b were also identified. The most commum PFGE patterns were A1 and A8. Analysis by PFGE and phage typing demonstrated that the most of the MDR were multiclonal and association among multiresistance, phage typing, and PFGE patterns was not so significant. Key words: Salmonella Typhimurium -phage types -multidrug-resistantSalmonella is one of the most important causal agents of foodborne illness. It can be isolated from a variety of foodstuff such as raw meats, poultry and poultry products, milk and milk products (Gorman & Adley 2004). The zoonotic nature of Salmonella infections is well established and the observed increase in human cases has been associated with high levels of infections in animal husbandry, mainly in pigs and poultry (Seyfarth et al. 1997).The Salmonella genus comprises more than 2500 serotypes (Popoff 2001), most of which are considered potential human pathogens, but only a reduced number of them have been associated with human infections (Uzzau et al. 2000). In developed countries, outbreaks of nontyphoidal Salmonella infections have been caused mainly by serotypes Enteritidis and Typhimurium (Seyfarth et al. 1997, Davis et al. 1999, Kariuky et al. 1999.S. Typhimurium is a common cause of human salmonellosis in many countries , Leegaard et al. 2000, Esaki et al. 2004, Martinez-Urtaza et al. 2004, Gorman & Adley 2004. It represents the most common serotype isolated from humans and animals in the United States (Bender et al. 2001, Rabatsky et al. 2004) and the second in the United Kingdom (Martinez-Urtaza et al. 2004).Studies reported in the state of São Paulo, Brazil, have also indicated that S. Typhimurium is the second most common serotype isolated from human sources (Tavechio et al.1996) and one of the most frequent from non-human sources (Taunay et al. 1996). The extensive use of antimicrobials in human and veterinary medicine has led to an increase in multidrug resistance strains (MDR) (Threfall et al. 1993, Glynn et al. 1998, Davis et al. 1999, Rabatsky et al. 2004. Fernandes et al. (1992) of S. Typhimurium strains isolated from humans presented high levels of resistance to antimicrobial agents (5-14 marks) and most were isolated in hospital environments, which have been considered the principal vehicle of epidemic MDR S. Typhimurium strains (Riley et al. 1984, Fernandes et al. 1992, Asensi et al. 1995, Kariuky...
Salmonellosis ranks among the most frequently reported zoonosis worldwide and is often associated with foodborne outbreaks. Since the 1950s, the distribution of Salmonella serotypes in Sao Paulo State, Brazil, has been documented and periodically reported. In this study, we updated the data on the distribution of Salmonella serotypes received in our reference laboratory, isolated from human infections and nonhuman sources, from 2004 to 2020. In that period, a total of 9,014 Salmonella isolates were analyzed, of which 3,553 (39.4%) were recovered from human samples, mainly of stool (65%) and blood (25.6%), and 5,461 (60.6%) were isolated from nonhuman origins, such as animals (47.2%), food (27.7%) and animal environments (18.6%). In human isolates, a total of 104 serotypes were identified and the most frequent ones were Enteritidis, Typhimurium, S. I. 4,[5],12:i:-, Dublin and Typhi. A consistent reduction of the Enteritidis proportion was observed over the years. Among the 156 serotypes identified in isolates with nonhuman origins, Enteritidis, Mbandaka, Typhimurium, Agona and Anatum were ranked as the top five Salmonella serotypes; in more recent years, S. Heidelberg has increased in frequency. Although with different proportions, the top 10 prevalent serotypes were identified in both human and nonhuman origins, underscoring the role of animals, food products and environment as reservoirs of Salmonella with potential to cause human salmonellosis.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.