Harmful algae blooms (HABs), which produce lethal toxins, are a growing global concern since they negatively affect the quality of drinking water and have major negative impact on wildlife, the fishing industry, as well as tourism and recreational water use. The goldstandard process employed in the field to identify and enumerate algae requires highly trained professionals to manually observe algae under a microscope, which is a very time-consuming and tedious task. Therefore, an automated approach to identify and enumerate these micro-organisms is much needed. In this study, we investigate the feasibility of leveraging machine learning and fluorescence-based spectral-morphological features to enable the identification of six different algae types in an automated fashion. More specifically, a custom multi-band fluorescence imaging microscope is used to capture fluorescence imaging data of a water sample at six different excitation wavelengths ranging from 405 nm -530 nm. Automated data processing and segmentation was performed on the captured fluorescence imaging data to isolate different micro-organisms from the water sample. A number of morphological and spectral fluorescence features are then extracted from the isolated micro-organism imaging data, and used to train neural network classification models designed for the purpose of identification of the six algae types given an isolated micro-organism. Experimental results using three different neural network classification models (one trained on morphological features, one trained on fluorescencebased spectral features, and one trained on fluorescencebased spectral-morphological features) showed that the use of either fluorescence-based spectral features or fluorescence-based spectral-morphological features to train neural network classification models led to statistically significant improvements in identification accuracy when compared to the use of morphological features (with average identification accuracies of 95.7% ± 3.5% and 96.1% ± 1.5%, respectively). These preliminary results are quite promising, given that the identification accuracy of human taxonomists are typically between the range of 67% and 83%, and thus illustrates the feasibility of leveraging machine learning and fluorescence-based spectral-morphological features as a viable method for automated identification of different algae types.
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