Enhanced biological phosphorus removal (EBPR) activated sludge process of municipal sewage treatment in warm climate could deteriorate supposedly due to phosphorus accumulating organisms (PAO) and glycogen accumulating organisms (GAO) competition for carbon substrate. Most studies on this topic employed the laboratory-scale sequencing batch reactor (SBR) process fed with synthetic substrate. This manuscript presents the experimental results using laboratory-scale modified Bardenpho process with real municipal sewage at a temperature range of 30±1°C without pH control. Process analysis especially on EBPR and mass balance of COD, nitrogen and phosphorus illustrated that phosphorus release and SCOD uptake in the anaerobic reactor and phosphorus uptake in the aerobic reactors were regularly observed. However, there were little SCOD uptake by PAO in the anaerobic reactor, while the major portion was utilized for denitrification, which resulted in poor EBPR. These results provided useful information for the design of further experimental studies.
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