Escherichia coli remain significant problem caused urinary tract infection; Results indicated a higher urinary tract infection in women compared with men across all age groups. Phylogenetic analysis showed that majority of uropathogenic isolated E. coli belong to phylogroup B2 followed by D. The isolates showed the existence of the first type of fimbriae, maximum P fimbriae positive isolates 74/94 (80.43%) were widely correlating with common UTI. colony adhesion factor (CAFÐ) represented in 40 (43.47%) and 5 (5.43%) colony adhesion factor (CAFØ). Six major clusters (A-F) were identified depending on antibiograms typing. B2 is the most phylogenetic type showed wide range of resistant from 1 to 12 resistant to antibiotic of remaining strains. 14 isolates (15.21%) were detected as ESâLs producers and 30 isolates (32.60%) of them were AmpC b-lactamases producers. prevalence of virulent genes occurred in 51 papC (55.43%), 66 fim H (71.73%) and SfaDE detected in low occurrence 21(22.82). These results emphasize the low or moderate resistant to antibiotics focused in isolates with high genetic content .These results indicated that the greater the resistance to antibiotics the less the genetic expression of the virulence factors, this confirms the reverse relationship. The genes required for uropathogenicity in a single isolate may not reflect virulence in another isolate. Pathogenicity is a multi-factor characteristic, the result of adhesion-related genes which interact in separate set in various genetic backgrounds.
Background: Acinetobacter baumannii has arisen as disturbing nosocomial pathogens between Iraqi hospitals inpatients.The aim of current study was to determine if the increase of A.baumannii incidence in patients on blaOXA-51 gene in A. baumannii that carry QS gene showed pathogenicity of clinical isolates and to determine the efficiency of ERIC-PCR fingerprinting method for genotyping of A. baumannii. Methods: Sixteen isolates diagnosed as A. baumannii and genetically confirmed by blaOXA-51 as a marker gene from different clinical sources in Baghdad hospitals.The virulence of the A. baumannii does not require, carrying full set of QS ( LasRand RhlI, LasI, RhlR) genes.Results: The positive QS genes results were distributed from high to low expression, lasI 75%(45/60),70%(42/60)for RhIR,50%(30/60) for rhII, and 13.3%(8/60)for LasR. Using fingerprinting ERIC-PCR analysis, 57isolates of A. baumannii were clustered into 2 groups while the remaining 3 were single isolates. The genetic linking of A. baumannii isolated from different hospitals inpatients was high, indicating horizontal gene transfers within hospitalized patients. Conclusion: Our findings indicated accurate and fast diagnosis method to detect virulent A. baumannii isolates harboring differing sets of QS by using blaOXA-51 gene and ERIC-PCR for genetic variations, respectively, possible to be helpful with epidemic infections.
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