Multiple evidences suggest that mitochondrial dysfunction is implicated in the pathogenesis of Parkinson’s disease via the selective cell death of dopaminergic neurons, such as that which occurs after prolonged exposure to the mitochondrial electron transport chain (ETC) complex I inhibitor, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyrine (MPTP). However, the effects of chronic MPTP on the ETC complexes and on enzymes of lipid metabolism have not yet been thoroughly determined. To face these questions, the enzymatic activities of ETC complexes and the lipidomic profile of MPTP-treated non-human primate samples were determined using cell membrane microarrays from different brain areas and tissues. MPTP treatment induced an increase in complex II activity in the olfactory bulb, putamen, caudate, and substantia nigra, where a decrease in complex IV activity was observed. The lipidomic profile was also altered in these areas, with a reduction in the phosphatidylserine (38:1) content being especially relevant. Thus, MPTP treatment not only modulates ETC enzymes, but also seems to alter other mitochondrial enzymes that regulate the lipid metabolism. Moreover, these results show that a combination of cell membrane microarrays, enzymatic assays, and MALDI-MS provides a powerful tool for identifying and validating new therapeutic targets that might accelerate the drug discovery process.
One of the aspects to consider during high-voltage (HV) equipment design is the reduction in the probability of corona effect onset. Indeed, the corona effect is related to high electric field values beyond the equipment’s insulation levels and insulation strength, among other factors. This issue can be addressed during the design step, either by modifying the geometry of the electrical device or by including additional elements in the equipment structure to smooth out the voltage gradient along critical regions, such as anti-corona devices. The study of anti-corona devices for HV insulators is well documented, in contrast to substation connectors. Therefore, the present study proposed the design of a novel anti-corona device for HV substation connectors, including a method for the selection of its dimensions. This study shows that the relationship between the dimensional design variables and the critical electrical field on the connector is described by linear and rational functions. Thus, the design process times are cut down due to a reduction in the number of simulations required to run the assessment of the anti-corona device arrangement impact.
Airearen ionizazioa dela eta, tentsio altuko lineetan koroa-efektua sortzen da, eta, ondorioz, elektrizitate-galerak eta lineetan kalteak gertatzen dira. Hortaz, artikulu honen helburua koroa-efektua eta haren detekzioa aztertzea da korronte alternoko goi-tentsioko sistemetan. Ikerketa-metodologiak Jorgensen eta Pedersen-ek lortutako datu esperimentalak erabili ditu, eta kalkuluak Townsend-en teorian oinarrituta daude. COMSOL multiphysics softwarea erabili da eremu elektrikoaren kalkulurako, beharrezko parametroa baita koroa-efektuaren kalkuluan. Lortutako emaitzak MATLAB bidez aztertu dira. Behin metodologia egiaztatuta, presioa, tenperatura, geometria eta simulazio-inguruneak koroa-efektuan duten eragina aztertu dira.
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