Kanadese vlier sabugueiro do Canada sauco del Canada Sambucus canadensis American elder(berry), bourtree, Canada elder, eastern elderberry, elderberry, common elder, sweet elder, pie elder, elder-blow, blackberry elder, Mexican elder sureau blanc, sureau du Canada, sirop blanc D. CHARLEBOIS, P. 1. BYERS, C. E. FINN, AND A. 1. THOMAS almindelig hyld Flieder, memeine Holunder, Holunder, schwarzer Flieder, schwarzer Holunder fekete bodza sambuco, sambuco nero, sambuco nostrale, zambuco, zambuco arboreo bez czarny sabugueiro, sabugueiro negro, sabugueiro preto 4epeH 6b3

No abstract

American (Sambucus canadensis L.) elderberry genotypes were evaluated at multiple locations, whereas European (S. nigra L.) elderberry genotypes were evaluated at a single location to assess genotypic differences and, for genotypes evaluated at multiple locations, to determine genotype × environment interactions (G × E). Seventeen S. canadensis genotypes were planted in replicated trials at Missouri State University (Mountain Grove, MO) and at the University of Missouri (Mt. Vernon, MO) or at the U.S. Department of Agriculture–Agricultural Research Service in Oregon (Corvallis). ‘Johns’, ‘Netzer’, ‘Adams II’, and ‘Gordon B’ were in common at all locations. In addition, three genotypes of S. nigra, which are not winter-hardy in Missouri, were planted in Oregon. All plants were established in 2003 and evaluated in 2004, 2005, and, for some traits, in 2006. Plants were evaluated for phenology (e.g., dates of budbreak, first flowering, full flowering, and first ripening), vegetative growth (e.g., number of shoots and plant height), yield components (e.g., total yield, number of cymes, cyme weight, and berry weight), and for pest incidence (e.g., eriophyid mites). For the genotypes in common to all locations, there were significant differences resulting from genotype, location, year, and the interactions for various traits. Although the trend was for Corvallis to have the highest and Mt. Vernon the lowest yield, there was no significant location effect. The significant genotype × environment interaction appeared to be primarily the result of the differential performance of ‘Johns’, which was generally high-yielding in Corvallis and low-yielding at both Missouri locations. The significant G × E suggests that as the Missouri institutions develop new cultivars, it will be important to test them individually at other locations and not rely on their relative performance compared with standards in Missouri. For the genotypes in common to the two Missouri sites, there was significant variation for many traits. Although there were no differences among genotypes for yield across the locations, there was a significant G × E. Although there were some small changes in performance among the sites for yield, the most dramatic changes were for ‘Wyldewood 1’ that was the second highest yielding genotype at Mountain Grove and the second worst at Mt. Vernon. Plant growth in Oregon was 40% and 60% greater than at Mountain Grove and Mt. Vernon, respectively, when the plants were first measured. In Oregon, the two Sambucus species behaved differently. Phenologically, although the S. nigra genotypes flowered ≈3 weeks earlier than the S. canadensis genotypes, they ripened at the same time, thereby shortening their exposure to potential biotic and abiotic stress. ‘Johns’, ‘York’, ‘Golden’, and ‘Gordon B’ were the highest yielding S. canadensis genotypes and ‘Korsør’ the highest of the S. nigra genotypes. Although ‘Korsør’ is considered high-yielding in Denmark, it did not yield as well as the highest yielding S. canadensis cultivars.

Itachi, a soybean (Glycine max IL.1 Meff.) variety with 0.2% normal seed urease activity, was recovered from a screen of 6,000 entries in the United States Department

BACKGROUND Elderberry (Sambucus spp.) fruit are used for food and dietary supplements in Europe and North America, and contain large amounts of cyanidin-based anthocyanins and other phenolics that may benefit human health. OBJECTIVES Information on the effect of both genotype and production environment on elderberry juice characteristics is needed in order to optimize production of quality food and dietary supplements. METHODS The characteristics of elderberry fruits relative to genetic and production environment were evaluated from 12 American elderberry genotypes at three U.S. sites (two in Missouri and one in Oregon) over three growing seasons. Additional genotypes of American and European elderberry were studied at the Oregon site. RESULTS Location, genotype, and growing season influenced pH, soluble solids, titratable acidity, total phenolics, and total anthocyanins. Elderberries grown in Oregon were consistently higher in acidity than those grown in Missouri. Differences in acidity and anthocyanin with environment were dependent on genotype. Non-acylated anthocyanins and flavonol-glycosides were more influenced by location than by genotype. CONCLUSION ‘Bob Gordon’ and ‘Adams 2’ genotypes, which are good producers in diverse environments, were significantly higher in total phenolic and total anthocyanin contents in all locations, and may be good selections for producing juices, wines, or health products.

Aims Elderberry (Sambucus spp.) is one of the oldest medicinal plants noted for its cardiovascular, anti-inflammatory, and immune-stimulatory properties. In this study, we investigated the anti-inflammatory and anti-oxidant effects of the American elderberry (Sambucus nigra subsp. canadensis) pomace as well as some of the anthocyanins (cyanidin chloride and cyanidin 3-O-glucoside) and flavonols (quercetin and rutin) in bv-2 mouse microglial cells. Main methods The bv-2 cells were pretreated with elderberry pomace (extracted with ethanol or ethyl acetate) or its anthocyanins and flavonols and stimulated by either lipopolysaccharide (LPS) or interferon-γ (IFNγ). Reactive oxygen species (ROS) and nitric oxide (NO) production (indicating oxidative stress and inflammatory response) were measured using the ROS detection reagent DCF-DA and the Griess reaction, respectively. Key findings Analysis of total monomeric anthocyanin (as cyanidin 3-O-glucoside equivalents) indicated five-fold higher amount in the freeze-dried ethanol extract as compared to that of the oven-dried extract; anthocyanin was not detected in the ethyl acetate extracts. Elderberry ethanol extracts (freeze-dried or oven-dried) showed higher anti-oxidant activities and better ability to inhibit LPS or IFNγ-induced NO production as compared with the ethyl acetate extracts. The phenolic compounds strongly inhibited LPS or IFNγ-induced ROS production, but except for quercetin, they were relatively poor in inhibiting NO production. Significance These results demonstrated difference in anti-oxidative and anti-inflammatory effects of elderberry extracts depending on solvents used. Results further identified quercetin as the most active component in suppressing oxidative stress and inflammatory responses on microglial cells.

No abstract

Elderberry (Sambucus spp.) juice contains a variety of polyphenols, mostly anthocyanins. In order to understand the variation of polyphenol levels by genotype, various elderberry juice samples were analyzed for total phenolics (TP), total monomeric anthocyanins (TMA) and individual anthocyanin content. The Folin-Ciocalteu total phenolic method and pH differential method were used to measure the TP and TMA content, respectively. The TP and TMA concentrations of elderberry were found to vary greatly among different genotypes. TMA content varied from 2.1% for ‘Sperandio’ to 60.6% for the ‘Bob Gordon’ cultivar. In addition, ultra-performance liquid chromatography with triple quadrupole mass spectrometry was used to separate and detect individual anthocyanins from samples prepared by solid phase extraction. Multiple-reaction-monitoring was used to process data for the reduction of false positives, maximizing selectivity, and reliable quantification. The quantitative performance of the method was validated, and a detection limit of 0.3 ng·ml−1 for cyanidin 3-O-glucoside was determined. This newly developed method may serve to characterize and profile various anthocyanins in elderberry juices for quality control, assessment of dietary intake, and anthocyanin-based biomedical studies.