Kanadese vlier sabugueiro do Canada sauco del Canada Sambucus canadensis American elder(berry), bourtree, Canada elder, eastern elderberry, elderberry, common elder, sweet elder, pie elder, elder-blow, blackberry elder, Mexican elder sureau blanc, sureau du Canada, sirop blanc D. CHARLEBOIS, P. 1. BYERS, C. E. FINN, AND A. 1. THOMAS almindelig hyld Flieder, memeine Holunder, Holunder, schwarzer Flieder, schwarzer Holunder fekete bodza sambuco, sambuco nero, sambuco nostrale, zambuco, zambuco arboreo bez czarny sabugueiro, sabugueiro negro, sabugueiro preto 4epeH 6b3
Itachi, a soybean (Glycine max IL.1 Meff.) variety with 0.2% normal seed urease activity, was recovered from a screen of 6,000 entries in the United States Department
Aims Elderberry (Sambucus spp.) is one of the oldest medicinal plants noted for its cardiovascular, anti-inflammatory, and immune-stimulatory properties. In this study, we investigated the anti-inflammatory and anti-oxidant effects of the American elderberry (Sambucus nigra subsp. canadensis) pomace as well as some of the anthocyanins (cyanidin chloride and cyanidin 3-O-glucoside) and flavonols (quercetin and rutin) in bv-2 mouse microglial cells. Main methods The bv-2 cells were pretreated with elderberry pomace (extracted with ethanol or ethyl acetate) or its anthocyanins and flavonols and stimulated by either lipopolysaccharide (LPS) or interferon-γ (IFNγ). Reactive oxygen species (ROS) and nitric oxide (NO) production (indicating oxidative stress and inflammatory response) were measured using the ROS detection reagent DCF-DA and the Griess reaction, respectively. Key findings Analysis of total monomeric anthocyanin (as cyanidin 3-O-glucoside equivalents) indicated five-fold higher amount in the freeze-dried ethanol extract as compared to that of the oven-dried extract; anthocyanin was not detected in the ethyl acetate extracts. Elderberry ethanol extracts (freeze-dried or oven-dried) showed higher anti-oxidant activities and better ability to inhibit LPS or IFNγ-induced NO production as compared with the ethyl acetate extracts. The phenolic compounds strongly inhibited LPS or IFNγ-induced ROS production, but except for quercetin, they were relatively poor in inhibiting NO production. Significance These results demonstrated difference in anti-oxidative and anti-inflammatory effects of elderberry extracts depending on solvents used. Results further identified quercetin as the most active component in suppressing oxidative stress and inflammatory responses on microglial cells.
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